Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Quantitative real-time PCR assays for the concurrent diagnosis of infectious laryngotracheitis virus, Newcastle disease virus and avian metapneumovirus in poultry

  • Mo, Jongseo (Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia) ;
  • Angelichio, Michael (IDEXX Laboratories, Inc.) ;
  • Gow, Lisa (IDEXX Laboratories, Inc.) ;
  • Leathers, Valerie (IDEXX Laboratories, Inc.) ;
  • Jackwood, Mark W. (Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, University of Georgia)
  • Received : 2021.05.25
  • Accepted : 2021.12.12
  • Published : 2022.03.31
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Abstract

Newcastle disease (ND), infectious laryngotracheitis (ILT) and avian metapneumovirus (aMPV) can be similar making it critical to quickly differentiate them. Herein, we adapted pre-existing molecular-based diagnostic assays for NDV and ILTV, and developed new assays for aMPV A and B, for use under synchronized thermocycling conditions. All assays performed equivalently with linearity over a 5 log10 dynamic range, a reproducible (R2 > 0.99) limit of detection of ≥ 10 target copies, and amplification efficiencies between 86.8%-98.2%. Using biological specimens for NDV and ILTV showed 100% specificity. Identical amplification conditions will simplify procedures for detection in diagnostic laboratories.

Keywords

Acknowledgement

This research was funded in part by the Veterinary Medical Experiment Station, UGA and by IDEXX Laboratories, Inc. (1021RR188-296).

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