The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Study of the Effect and Underlying Mechanism of Clove Extract on Monosodium Iodoacetate-Induced Osteoarthritis in Rats

정향(丁香) 추출물이 골관절염 흰쥐에 미치는 효과 및 기전 연구

  • Jin A, Lee (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Min Ju, Kim (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Wook, Seo (Dept. of Rehabilitation Sciences, Graduate School, Daegu University) ;
  • Mi-Rae, Shin (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서성욱 (대구대학교 대학원 재활과학과) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2022.11.21
  • Accepted : 2022.12.21
  • Published : 2022.12.30
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Abstract

Objective: The aim of this study was to identify the efficacy and underlying mechanism of cloves as an osteoarthritis (OA) treatment in a monosodium iodoacetate (MIA)-induced rat OA model. Osteoarthritis (OA) is nowadays one of the most prevalent degenerative joint diseases. Methods: Sprague-Dawley rats treated with MIA (50 μL; 80 mg/mL) were used as in vivo OA models. Cloves (100 and 200 mg/kg b.w.) were administered orally once daily for 2 weeks from 7 days after MIA injection. Changes in hindpaw weight distribution (HWD) were measured as a joint discomfort index. Activation markers related to inflammatory responses and cartilage degeneration in the right knee joints were evaluated by serum analysis and western blotting. Results: HWD decreased in the MIA control group but showed a dose-dependent elevation after clove treatment. Clove treatment inhibited inflammatory factors by PI3K/Akt/NF-κB signaling pathways, while also activating antioxidant factors through Sirt1/AMPK signaling pathways. Clove treatment also suppressed matrix metalloproteinase (MMP) overexpression and significantly increased the levels of tissue inhibitors of metalloproteinases (TIMPs). Conclusions: Treatment with cloves effectively reversed MIA-induced effects. Therefore, clove treatment could have the potential to protect against or treat OA.

Keywords

Acknowledgement

이 연구는 2022년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다(No. 2018R1A5A2025272와 No. 2021R1I1A1A01059605).

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