DOI QR코드

DOI QR Code

제2형 당뇨 동물모델에서 일반메밀과 쓴메밀의 항당뇨 효과 비교

Anti-diabetic effects of common buckwheat and tartary buckwheat in type II diabetes animal model

  • 김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 손황배 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 최지명 (부산대학교 식품영양학과 및 김치연구소) ;
  • 조은주 (부산대학교 식품영양학과 및 김치연구소) ;
  • 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 서종택 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 장동칠 (바이오에너지 작물연구소) ;
  • 김율호 (농촌진흥청 국립식량과학원 중부작물과)
  • Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Nam, Jung Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jong Nam (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Chang, Dong Chil (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yul Ho (Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration)
  • 투고 : 2021.09.25
  • 심사 : 2021.12.13
  • 발행 : 2022.02.28

초록

메밀은 전 세계적으로 곡식, 싹, 차 등 다양한 형태로 이용되고 있으며, 주요 재배종으로는 일반메밀과 쓴메밀이 있다. 본 연구는 일반메밀과 쓴메밀 종실과 전초를 대상으로 항산화 및 항당뇨 활성 효과를 평가하였다. 루틴 함량은 쓴메밀 추출물이 일반메밀 추출물보다 44-48배 이상 높았으며, 퀘세틴은 전초에서만 검출되었다. 플라보노이드 및 폴리페놀 함량도 쓴메밀 추출물이 일반메밀 추출물보다 2.5-4.8배 높았다. STZ 투여로 제2형 당뇨가 유발된 동물모델에 일반메밀과 쓴메밀의 종실과 전초를 급여하여 공복혈당, 경구 내당능, 인슐린 내성 변화, 혈중 포도당 및 인슐린 농도 등을 통해 항당뇨 효과를 확인하였다. 종실의 경우는 일반메밀에 비해 쓴메밀 종실군에서 경구 내당능 효과가 뛰어났으며, 혈청 포도당 농도도 유의적으로 감소하는 것을 확인하였다. 전초의 경우, 일반메밀이나 쓴메밀 급여 실험군에서 모두 당뇨대조군에 비하여 유의적인 항당뇨 효과를 보였으며, 인슐린 저항성 지표인 HOMA-IR과 인슐린 민감성 지표인 QUICKI를 비교해본 결과에서도 종실과 전초 섭취군 모두 인슐린 저항성을 감소시키고, 인슐린 민감성을 증가시키는 것을 확인하였다. 이러한 결과들을 고려하면 루틴 함량 및 항산화활성이 쓴메밀 전초의 경우 항당뇨 효과와 밀접한 관계가 있으며, 루틴 함량과 항산화 활성이 높은 쓴메밀 종실 및 일반메밀과 쓴메밀 전초는 항당뇨 식의약 소재로 활용 가능성이 높은 것으로 판단된다.

In this study, we evaluated the antioxidant and antidiabetic effects of buckwheat. The diabetic animal models were divided into four groups: normal mice group (NOR), streptozotocin-induced diabetic mice group (STZ), group treated with seeds of common or tartary buckwheat (SCB or STB), and the group treated with whole plants of common or tartary buckwheat (PCB or PTB). Rutin content was 44-48 times higher in STB or PTB than in SCB. Oral glucose tolerance and insulin resistance were significantly reduced by treatment with STB, PCB, and PTB. Treatment with PTB also decreased the serum glucose level significantly and the serum insulin levels slightly compared with the STZ group. These results suggest that rutin content and antioxidant activity are closely related to the antidiabetic effect of the treatment. Our results demonstrate that the seeds of tartary buckwheat and whole plants of either common or tartary buckwheat have antidiabetic effects-attenuating blood glucose in an animal model of type II diabetes.

키워드

과제정보

본 논문은 농촌진흥청 작물시험연구사업(연구과제: 루틴 고함유 쓴메밀 우량계통 육성 및 이용성 증진 연구, 세부과제: 쓴메밀 가공적성 평가 및 이용 기술 개발, PJ01189403)에 의해 이루어진 것이며 이에 감사드립니다.

참고문헌

  1. Adeghate E, Parvez SH. Nitric oxide and neuronal and pancreatic beta cell death. Toxicol. 153: 143-156 (2000) https://doi.org/10.1016/S0300-483X(00)00310-3
  2. Ahmed OM, Moneim AA, Yazid LA, Mahmoud AM. Antihyperglycemic, antihyperlipidemic and antioxidant effects and the probable mechanisms of action of Ruta graveolens infusion and rutin in nicotinamide-streptozotocin-induced diabetic rats. Diabetol. Croat. 39: 15-35 (2010)
  3. Akondi RB, Kumar P, Annapurna A, Pujari M. Protective effect of rutin and naringin on sperm quality in streptozotocin (STZ) induced type 1 diabetic rats. Iran. J. Pharm. Res. 10: 585 (2011)
  4. Alinejad B, Ghorbani A, Sadeghnia HR. Effects of combinations of curcumin, linalool, rutin, safranal, and thymoquinone on glucose/serum deprivation-induced cell death. Avicenna J. Phytomed. 3: 321 (2013)
  5. Cai EP, Lin JK. Epigallocatechin gallate (EGCG) and rutin suppress the glucotoxicity through activating IRS2 and AMPK signaling in rat pancreatic β cells, J. Agric. Food Chem. 57: 9817-9827 (2009) https://doi.org/10.1021/jf902618v
  6. Campbell CG. Buckwheat Fagopyrum esculentum Moench. Plant Genet. Resour., CAB International. pp. 3-93 (1997)
  7. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  8. Dewanto V, Xianzhong W, Liu RH. Processed sweet corn has higher antioxidant activity. J. Agr. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  9. Faerch K, Borch-Johnsen K, Holst JJ, Vaag A. Pathophysiology and aetiology of impaired fasting glycaemia and impaired glucose tolerance: does it matter for prevention and treatment of type 2 diabetes? Diabetol. 52: 1714-1723 (2009) https://doi.org/10.1007/s00125-009-1443-3
  10. Fernandes AA, Novelli EL, Okoshi K, Okoshi MP, Di Muzio BP, Guimaraes JF, Fernandes Junior A. Influence of rutin treatment on biochemical alterations in experimental diabetes. Biomed Pharmacother 64: 214-219 (2010) https://doi.org/10.1016/j.biopha.2009.08.007
  11. Folin O, Denis W. On phosphotungstic phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912) https://doi.org/10.1016/S0021-9258(18)88697-5
  12. Ghorbani A. Mechanisms of antidiabetic effects of flavonoid rutin. Biomed. Pharmacother. 96: 305-312 (2017) https://doi.org/10.1016/j.biopha.2017.10.001
  13. Gordon T, Castelli WP, Hjortland M, Kannel WB, Dawber TR. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am. J. Med. 62: 707-714 (1977) https://doi.org/10.1016/0002-9343(77)90874-9
  14. Hosseini A, Shafiee-Nick R, Ghorbani A. Pancreatic beta cell protection/regeneration with phytotherapy. Braz. J. Pharm. Sci. 51: 1-16 (2015) https://doi.org/10.1590/S1984-82502015000400001
  15. Hue JJ, Kim JS, Kim JH, Nam SY, Yun YW, Jeong JH, and Lee BJ. Antiglycemic effect of carnosine in diabetic mice J. Fd. Hyg. Safety 24: 391-397 (2009)
  16. Hwang JY, Han JS. Inhibitory effects of Sasa borealis leaves extracts on carbohydrate digestive enzymes and postprandial hyperglycemia. J. Korean Soc. Food Sci. Nutr. 36: 989-994 (2007) https://doi.org/10.3746/JKFN.2007.36.8.989
  17. Hwang EJ, Lee SY, Kwon SJ, Park MH, Boo HO. Antioxidative, antimicrobial and cytotoxic activities of Fagopyrum esculentum Moench extract in germinated seeds. J. Korean Medicinal Crop Sci. 4: 1-7 (2006)
  18. Janbaz KH, Saeed SA, Gilani AH. Protective effect of rutin on paracetamol-and CCl 4-induced hepatotoxicity in rodents. Fitoterapia 73: 557-563 (2002) https://doi.org/10.1016/S0367-326X(02)00217-4
  19. Jeon YH, Moon JW, Kweon HJ, Jeoung YJ, An CS, Jin HL, Hur SJ, Lim BO. Effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention of diabetic cataract and retinopathy in streptozotocin-induced diabetes rat model. Korean J. Medicinal Crop Sci. 18: 15-21 (2010)
  20. Kamalakkannan N, Prince PSM. Rutin improves the antioxidant status in streptozotocin-induced diabetic rat tissues, Mol. Cell. Biochem. 293: 211-219 (2006) https://doi.org/10.1007/s11010-006-9244-1
  21. Kappel VD, Cazarolli LH, Pereira DF, Postal BG, Zamoner A, Reginatto FH, Silva FRMB. Involvement of GLUT-4 in the stimulatory effect of rutin on glucose uptake in rat soleus muscle. J. Pharm. Pharmacol. 65: 1179-1186 (2013) https://doi.org/10.1111/jphp.12066
  22. Khan A, Pessin J. Insulin regulation of glucose uptake: a complex interplay of intracellular signalling pathways. Diabetol. 45: 1475-1483 (2002) https://doi.org/10.1007/s00125-002-0974-7
  23. Khaki A, Fathiazad F, Ahmadi-Ashtiani HR, Rezazadeh SH, Rastegar H, Imani AM. Compartments of quercetin & allium cepa (onion) on blood glucose in diabetic rats. J Med Plants (Suppl 6) 9: 107-12 (2010)
  24. Kim HA. Isolation and characterization of antidiabetic compounds from buckwheat, Department of food science and technology Graduate School, Kunsan National University. Kunsan, Korea (2006)
  25. Kim SH, Hwang SY, Park OS, Kim MK, Chung YJ. Effect of Pinus densiflora extract on Blood glucose level, OGTT and biochemical parameters in streptozotocin induced diabetic rats. J Korean Soc. Food Sci. Nutr. 4: 973-979 (2005)
  26. Kim HB, Kim SL. Quantification and varietal variation of rutin in mulberry fruits. Korean J. Seric. Sci. 46: 1-5 (2004)
  27. Kim SJ, Kim YH. Agricultural guide Buckwheat. Rural Development Administration, Jeonju, Korea. pp. 7-90 (2018)
  28. Kim SL, Kim SK, Park CH. Introduction and nutritional evaluation of buckwheat sprouts as a new vegetable. Food Res. Int. 37: 319-327 (2004) https://doi.org/10.1016/j.foodres.2003.12.008
  29. Kim JH, Pan JH, Cho HT, Kim YJ. Black ginseng extract counteracts streptozotocin-induced diabetes in mice. PLoS One 11, doi:10.1371 (2016) https://doi.org/10.1371
  30. Kim SJ, HB Sohn, Hong SY, Lee JN, Kim KD, Suh JT, Nam JH, Chang DC, Park MW, Kim YH. Construction of data system on seed morphological traits and functional component in tartary buckwheat germplasm. Korean J. Plant Res. 33: 446-459 (2020) https://doi.org/10.7732/KJPR.2020.33.5.446
  31. Kim SJ, Sohn HB, Kim GH, Lee YY, Hong SY, Kim KD, Nam JH, Chang DC, Suh JT, Koo BJ, Kim YH. Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.) Korean J. Food Sci. Technol. 49: 258-264 (2017) https://doi.org/10.9721/KJFST.2017.49.3.258
  32. Kim SJ, Sohn HB, Lee KT, Shin JS, Kim SY, Nam JH, Hong SY, Suh JT, Chang DC, Kim YH. Anti-inflammatory effects of seed ethanolic extracts of the common buckwheat and tartary buckwheat are mediated through the suppression of inducible nitric oxide synthase and pro-inflammatory cytokines. in LPS-induced RAW 264.7 macrophage cells. Korean J. Food Sci. Technol. 51: 565-575 (2019) https://doi.org/10.9721/KJFST.2019.51.6.565
  33. Kreft M. Buckwheat phenolic metabolites in health and disease. Nutr. Res. Rev. 29: 30-39 (2016) https://doi.org/10.1017/S0954422415000190
  34. Kreft S, Janes D, Kreft I. The content of fagopyrin and polyphenols in common and tartary buckwheat sprouts. Acta Pharm. 63: 553-560 (2013) https://doi.org/10.2478/acph-2013-0031
  35. Kreft I, Zhou M, Golob A, Germ M, Likar M, Dziedzic K, Luthar Z. Breeding buckwheat for nutritional quality. Breed. Sci. 70: 67-73 (2020) https://doi.org/10.1270/jsbbs.19016
  36. Kwon HW, Koo GB, Lee YJ, Kim JH, Lee MH, In G. Effect of Korean red ginseng extract (KGC05P0) on regulating insulin sensitivity, insulin and blood glucose level in hyperinsulinemia type 2 diabetic mice. J. Korean Soc. Food Sci. Nutr. 49: 539-546 (2020) https://doi.org/10.3746/jkfn.2020.49.6.539
  37. Lee EJ, Choi H, Yoon WC, Kim YS, Song BN, Lee MY, Park BR, Lee SH, Choi JH, Park SY. Anti-diabetic and Lipid Profile Effect of Astragalus membranaceus (Fisch.) bunge fermented by Aspergillus awamori in db/db mice. Korean J. Medicinal Crop Sci. 29: 263-272 (2021) https://doi.org/10.7783/KJMCS.2021.29.4.263
  38. Lee KH, Ham HM, Kim HJ. Park HY, Sim EY, Oh SK, Kim WH, Jeong HS, Woo KS. Functional components and radical scavenging activity of germinated brown rice according to variety. Korean J. Food Nutr. 29: 145-152 (2016) https://doi.org/10.9799/KSFAN.2016.29.2.145
  39. Lee DG, Jang IS, Yang KE, Yoon SJ, Baek SJ, Lee JY, Suzuki T, Chung KY, Woo SH, Choi JS. Lee HH. A study on the utilization of functional cosmetics materials using the bioactive compounds from buckwheat. Kor. J. Aesthet. Cosmetol. 6: 1-8 (2008)
  40. Lee JS, Lee MH, Chang YK, Ju JS, Son HS. Effects of buckwheat diet on serum glucose and lipid metabolism in NIDDM. Korean J. Nutr. 28: 809-817 (1995)
  41. Lee SZ, Park SH, Lee HS. Change in vivo lipid peroxidation and antioxidant defense system in streptozotocin-induced diabetic rats: a time course study. Korean J. Nutr. 34: 253-264 (2001)
  42. Lee JS, Park SJ, Sung KS, Han CK, Lee MH, Jung CW, Kwon TB. Effects of germinated-buckwheat on blood pressure, plasma glucose and lipid levels of spontaneously hypertensive rats. Korean J. Food Sci. Technol. 32: 206-211 (2000)
  43. Lee MY, Shin IS, Jeon WY, Shin N, Shin HK. Bangpungtongseongsan, a traditional herbal medicine, attenuates chronic asthmatic effects induced by repeated ovalbumin challenge. Int. J. Mol. Med. 33: 978-986 (2014) https://doi.org/10.3892/ijmm.2014.1654
  44. Matkovics B, Kotorman M, Varga C. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol. Hung. 85: 29-38 (1998)
  45. Niture NT, Ansari AA, Naik SR. Anti-hyperglycemic activity of rutin in streptozotocin-induced diabetic rats: an effect mediated through cytokines, antioxidants and lipid biomarkers. Ind. J. Exp. Biol. 52: 720-727 (2014)
  46. Park SM. Strategies for development of anti-diabetic functional foods. Food Sci. Ind. 40: 46-58 (2007) https://doi.org/10.23093/FSI.2007.40.2.46
  47. Park HB, Jeong NH, Park IC. Studies on the purification and production of the D-pinitol and D-chiro-inositol from soybean and buckwheat. Ministry of Agriculture. pp. 2-77 (2004)
  48. Park CH, Kim YB, Choi YS, Heo K, Kim SL, Lee KC, Chang KJ, Lee HB. Rutin content in food products processed from groats, leaves, and flowers of buckwheat. Fagopyrum 17: 63-66 (2000)
  49. Park JE, Lee GH, Kim JH, Choi SW, Kim EJ. Anti-obesity effect of Ramulus mori extracts and stilbenes in high fat diet-fed C57BL/6J mouse. J. Nutr. Health 53: 570-582 (2020) https://doi.org/10.4163/jnh.2020.53.6.570
  50. Redondo MJ, Fain PR, Eisenbarth GS. Genetics of type 1A diabetes. Recent Prog. Horm. Res. 56: 69-89 (2001) https://doi.org/10.1210/rp.56.1.69
  51. Rhee SY, Woo TJ, Chon S, Hwang YC, Oh S, Ahn KJ, Chung HY, Kim SW, Kim JW, Kim YS. Characteristics of insulin resistance and insulin secretory capacity in Korean subjects with IFG and IGT. Diabetes Res. Clin. Pract. 89: 250-255 (2010) https://doi.org/10.1016/j.diabres.2010.05.002
  52. Sakihama Y, Cohen MF, Grace SC, Yamasaki H. Plant phenolic antioxidant and pro-oxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicol. 177: 67-80 (2002) https://doi.org/10.1016/S0300-483X(02)00196-8
  53. Son HK, Han JH, Lee JJ. Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocininduced diabetes. Korean J. Food Preserv. 21: 549-559 (2014) https://doi.org/10.11002/KJFP.2014.21.4.549
  54. Son HK, Lee YM, Park YH, Lee JJ. Effect of young barley leaf powder on glucose control in the diabetic rats. Korean J. Community Living Sci. 27: 19-29 (2016) https://doi.org/10.7856/kjcls.2016.27.1.19
  55. Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond. Am. J. Clin. Nutr. 81: 215S-217S (2005) https://doi.org/10.1093/ajcn/81.1.215S
  56. Stadtman ER, Berlett BS. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metab. Rev. 30: 325-243 (1998)
  57. Suk JH, Kim MK, Ju JW, Han JS, Park JH. The effect of green tea polyphenol on plasma glucose, lipid levels and antioxidant system in type 2 diabetes patients. J. Korean Diabetes Assoc. 30: 217-225 (2006) https://doi.org/10.4093/jkda.2006.30.3.217
  58. Vinayagam R, Jayachandran M, Xu B. Antidiabetic effects of simple phenolic acids: A comprehensive review. Phytother. Res. 30: 184-199 (2016) https://doi.org/10.1002/ptr.5528
  59. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutr. Metab. 23: 12-60 (2015)
  60. Wilcox G. Insulin and insulin resistance. Clin Biochem. Rev. 26(2): 19-39 (2005)
  61. Wolff SP, Jiang ZY, Hunt JV. Protein glycation and oxidative stress in diabetes mellitus and ageing. Free Radic. Biol. Med. 10: 339-352 (1991) https://doi.org/10.1016/0891-5849(91)90040-A
  62. Wu WL, Wang J, Qiu J, Li Z. The analysis of fagopyritols from tartary buckwheat and their anti-diabetic effects in KK-Ay type 2 diabetic mice and HepG2 cells. J. Functional Foods 50: 137-146 (2018) https://doi.org/10.1016/j.jff.2018.09.032
  63. Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWTFood Sci. Technol. 41(6): 1060-1066 (2008)
  64. Yoon BR, Cho BJ, Lee HK, Kim DJ, Rhee SK, Hong HD, Kim KT, Cho CW, Choi HS, Lee BY, Lee OH. Antioxidant and anti-adipogenic effects of ethanolic extracts from tartary and common buckwheats. Korean J. Food Preserv. 19: 123-130 (2012) https://doi.org/10.11002/KJFP.2012.19.1.123