한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제45권4호
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  • Pages.305-316
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  • 2022
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
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Prevalence of Bordetella bronchiseptica, Mycoplasma felis, and Chlamydia felis using a newly developed triplex real-time polymerase chain reaction assay in Korean cat population

  • Hye-Ryung, Kim (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Gyu-Tae, Jeon (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Jong-Min, Kim (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Ji-Su, Baek (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Yeun-Kyung, Shin (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Oh-Kyu, Kwon (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Hae-Eun, Kang (Foreign Animal Disease Division, Animal and Plant Quarantine Agency) ;
  • Ho-Seong, Cho (College of Veterinary Medicine, Jeonbuk National University) ;
  • Doo-Sung, Cheon (Postbio Inc.) ;
  • Choi-Kyu, Park (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)
  • 투고 : 2022.12.07
  • 심사 : 2022.12.08
  • 발행 : 2022.12.30
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초록

Bordetella (B.) bronchiseptica, Mycoplasma (M.) felis, and Chlamydia (C.) felis are considered as main bacterial pathogens of feline upper respiratory tract disease (URTD). In this study, a new triplex quantitative real-time polymerase chain reaction (tqPCR) assay was developed for the rapid and differential detection of these bacteria in a single reaction. The assay specifically amplified three bacterial genes with the detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 1%. Based on the diagnostic results of the assay using 94 clinical samples obtained from cats with URTD signs, prevalence of B. bronchiseptica, M. felis, or C. felis was 10.6%, 36.2%, or 6.4%, respectively, indicating that the diagnostic sensitivity was comparable to those of previously reported monoplex qPCR assays. The dual infection rates for B. bronchiseptica and M. felis or M. felis and C. felis was 2.1% or 3.2%, respectively. These results indicated that M. felis has been widely spread, and its co-infection with B. bronchiseptica or M. felis has been frequently occurred in Korean cat population. The developed tqPCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens and the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of feline URTD in Korea.

키워드

과제정보

This work was supported by the fund (Z-15430852022-23-03) by the Animal and Plant Quarantine Agency, Korea.

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