Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Antioxidant Activity of Honeydew Honey Produced by Apis mellifera L.

양봉꿀벌이 생산한 감로꿀의 항산화 활성

  • Se-Gun, Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Hyo-Young, Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Hong-Min, Choi (Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Hye-Jin, Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Sang-Mi, Han (Department of Agricultural Biology, National Institute of Agricultural Sciences)
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김효영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 최홍민 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 이혜진 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2022.10.07
  • Accepted : 2022.11.25
  • Published : 2022.12.01
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Abstract

To evaluate biological activity of honeydew honey produced by Apis mellifera L. in Korea, we measured antioxidant activity by using DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS [2,2-azobis(3-ethylbenzothiazoline-6-sulfonate)], FRAP (Ferric reducing antioxidant power), and total polyphenol content assays. Korean honeydew honey respectively scavenged 26% and 86% of DPPH and ABTS radicals at the highest concentration of 10 mg/mL. In the result of FRAP assay, Korean honeydew honey showed activity (126 µM of FRAP value) to reduce Fe3+ to Fe2+. Total polyphenol content was 73.41 mg GAE/kg. Korean honeydew honey exhibited excellent antioxidant activity due to having high radicals scavenging ability and reducing power of ferric ion as well as the presence of phenolic compounds. These findings suggest that Korean honeydew honey has great potential as a functional food material.

국내 생산 감로꿀의 기능성 식품 소재로 활용하기 위하여 DPPH (1,1-diphenyl-2-picryl hydrazyl)와 ABTS [2,2-azobis (3-ethylbenzothiazoline-6-sulfonate)] 라디칼 소거능, FRAP (Ferric reducing antioxidant power) 활성, 총 폴리페놀 함량 분석을 통하여 항산화 활성을 평가하였다. 국내 생산 감로꿀은 DPPH와 ABTS 라디칼 소거 활성을 보였으며 최고농도 10 mg/mL에서 DPPH에 비하여 ABTS 라디칼 소거능이 3배 이상 높게 측정되었다. FRAP 분석에서는 ferric ion (Fe3+)를 126 µM의 ferrous (Fe2+)로 환원시켰다. 또한, 국내 생산 감로꿀에 함유된 폴리페놀성 화합물의 총량은 73.41 mg GAE/kg로 확인되었다. 이상의 연구결과에 비추어 볼 때, 국내 생산 감로꿀의 우수한 라디칼 소거능과 환원력을 가지며 천연 항산화제로 알려진 페놀성 화합물을 존재로 인하여 항산화 활성을 가지는 것으로 확인되었다. 본 연구결과는 국내 생산 감로꿀의 기능성 식품 소재로 활용할 수 있는 기초자료로 제공하는 바이다.

Keywords

Acknowledgement

본연구는 농촌진흥청 공동연구사업(과제번호: PJ01574601)에 의하여 수행되었으므로 감사를 드립니다

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