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Analysis of Antioxidant Activity, Total Phenol Content, and Flavonoid Content of Chungtaejeon Tea Extracts

청태전(Chungtaejeon Tea) 추출물의 항산화 활성과 총 페놀 및 플라보노이드 함량 분석

  • GE, GE (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Dong-Geun, Han (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Hyun-Jeong, Kim (R&D Center, HONEST. Co., Ltd.) ;
  • Eun-Young, Choi (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Bong-Jeon, An (Department of Cosmeceutical Science, Daegu Hanny University)
  • 갈 격 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 한동근 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 김현정 ((주)허니스트) ;
  • 최은영 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2022.11.17
  • Accepted : 2022.12.18
  • Published : 2022.12.30

Abstract

In this study, antioxidant efficacy was analyzed to verify the value of use as an antioxidant-related functional cosmetic material using Cheongtaejeon tea extract. Cheongtaejeon tea was extracted, concentrated, and freeze-dried with hot water and 70% ethanol as a solvent to prepare samples, and then ABTS+ radical scavenging activity, electron donating ability, SOD-like ability, reducing power, ferric reducing antioxidant power (FRAP), total phenolic, and flavonoid contents were analyzed. In the ABTS+ radical scavenging activity experiment, both the Cheongtaejeon hydrothermal extract (CTW) and the Cheongtaejeon 70% ethanol extract (CTE) showed erasure activity of more than 98% at a concentration of 1,000 ㎍/mL, and in the electron donating analysis experiment, CTW and CTE, respectively, 42.20% and 78.82%. As a result of SOD-like activity measurement, activity of 39.73% and 67.39% of CTW and CTE, respectively, was confirmed. In the case of FRAP and reducing power experiments, both CTW and CTE showed high effects, and as a result of analyzing the total phenol and flavonoid contents, both CTW and CTE showed high contents. Based on the results of the experiment, the Cheongtaejeon tea extract is expected to have a highly valuable as a functional cosmetics material related to antioxidants.

본 연구에서는 청태전 추출물을 이용하여 항산화 관련 기능성 화장품 소재로서의 활용가치를 검증하고자 항산화 효능을 분석하였다. 열수와 70% 에탄올을 용매로 청태전을 추출, 농축, 동결건조하여 시료를 제조한 다음 ABTS+ radical 소거능, 전자공여능, SOD 유사 활성능, 환원력, FRAP 환원성 항산화활성, 총 페놀 및 플라보노이드 함량 분석을 진행하였다. ABTS+ radical 소거능 분석 실험의 경우, 청태전 열수 추출물(CTW), 청태전 70% 에탄올 추출물(CTE) 모두 1,000 ㎍/mL의 농도에서 98% 이상의 소거 활성을 나타내었으며, 전자공 여능 분석 실험의 경우, CTW, CTE 각각 42.20%, 78.82%의 활성을 나타내었다. SOD 유사 활성능 측정 결과, CTW, CTE 각각 39.73%, 67.39%의 활성이 확인되었다. FRAP, Reducing power 실험의 경우, CTW, CTE 모두 높은 효과를 나타내었고, 총 페놀 및 플라보노이드 함량 분석 결과, CTW, CTE 모두 높은 함량을 나타내었다. 따라서 청태전 추출물은 항산화 관련 기능성 화장품 소재로서 활용가치가 높을 것으로 사료된다.

Keywords

References

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