The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory Effects of Cistanche deserticola Water Extracts in LPS-stimulated RAW 264.7 Macrophages

육총용(肉蓯蓉) 열수추출물이 RAW 264.7 대식세포에 미치는 항염증 효과

  • Park, Sun-Myoung (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Lee, Kyou-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Hong, Chul-Hee (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
  • 박선명 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 이규영 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍철희 (상지대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2022.01.14
  • Accepted : 2022.02.12
  • Published : 2022.02.25
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Abstract

Objectives : The purpose of this study is to confirm the anti-inflammatory effects of Cistanche deserticola Water Extracts(CDWE). Methods : In this study, cell viability was determined by MTT assay. NO production was determined with Griess reagent, and IL-6 production was determined by ELISA. And western blot analysis was performed to confirm the protein expression of NOS2, COX-2, I𝜅B-𝛼, p-I𝜅B-𝛼, and NF-𝜅B. Results : CDWE was not cytotoxic at 15.625-1,000㎍/㎖ in RAW 264.7 cells. CDWE inhibited NO production in a concentration-dependent manner, with inhibitory effect on expression levels of IL-6. Expression level of NOS2, NF-𝜅B p65, and p-I𝜅B-𝛼 decreased at 250-1,000㎍/㎖. mRNA expression of COX-2 suppressed at 250, 1,000㎍/㎖. Conclusions : These results suggest that CDWE has anti-inflammatory effects and can be used as an anti-inflammatory therapeutic material.

Keywords

References

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