Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability

  • Yeou, Sanghun (Department of Physics, Pohang University of Science and Technology) ;
  • Lee, Nam Ki (Department of Chemistry, Seoul National University)
  • Received : 2021.07.09
  • Accepted : 2021.07.20
  • Published : 2022.01.31
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Abstract

The various DNA-protein interactions associated with the expression of genetic information involve double-stranded DNA (dsDNA) bending. Due to the importance of the formation of the dsDNA bending structure, dsDNA bending properties have long been investigated in the biophysics field. Conventionally, DNA bendability is characterized by innate averaging data from bulk experiments. The advent of single-molecule methods, such as atomic force microscopy, optical and magnetic tweezers, tethered particle motion, and single-molecule fluorescence resonance energy transfer measurement, has provided valuable tools to investigate not only the static structures but also the dynamic properties of bent dsDNA. Here, we reviewed the single-molecule methods that have been used for investigating dsDNA bendability and new findings related to dsDNA bending. Single-molecule approaches are promising tools for revealing the unknown properties of dsDNA related to its bending, particularly in cells.

Keywords

Acknowledgement

This work was supported by the Creative-Pioneering Researchers Program of Seoul National University and NRF-2020R1A5A1019141 and NRF-2019R1A2C2090896 of the National Research Foundation of Korea.

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