Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Effects of Oral Administered Hot Water Extracts of Korean Black Ginseng on Wound Healing in Mice

피부(皮膚) 창상(創傷) 동물모델에서 흑삼(黑蔘) 열수 추출물 경구 투여의 효과

  • Kim, Tae-Ryeong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Young-Jun (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University)
  • 김태령 (대구한의대학교 한의과대학 한방재활의학교실) ;
  • 김영준 (대구한의대학교 한의과대학 한방재활의학교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학교실)
  • Received : 2021.12.15
  • Accepted : 2021.12.23
  • Published : 2022.01.31
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Abstract

Objectives This study aims to evaluate the wound healing effects of oral administered hot water extracts of Korean black ginseng (KBG). Methods 40 C57BL/6 mice were divided into five groups; normal, control, vitamin E 200 mg/kg, KBG 100 mg/kg, KBG 200 mg/kg, each n=8. Skin wounds were made in the back of all mice except normal group using biopsy punches. Wounds were observed on days 7 and 14 after injury. The anti-oxidant and inflammatory protein levels were evaluated using western blotting. Skin tissue was analyzed by hematoxylin & eosin and Masson's trichrome staining method. Results KBG significantly accelerated reducing wound area. KBG significantly decreased myeloperoxidase activity. KBG significantly decreased oxidative stress factors such as NADPH oxidase-4 and p22phox and increased antioxidant enzymes including nuclear factor erythroid 2-related factor2, kelch-like ECH-associated protein-1, heme oxygenase-1, superoxide dismutase, catalase and glutathione peroxidase-1/2. Moreover, KBG significantly decreased inflammation factors including nuclear factor-κB, phosphorylated inhibitor of κBα, cyclooxygenase-2, inducible nitric oxide synthase, tumor necrosis factor-α and interleukin (IL)-6 and increased anti-inflammation cytokine such as IL-4 and IL-10. In addition, KBG significantly increased tight junction proteins including claudin-1, claudin-3, claudin-4. In histopathologic, KBG made the epithelium thin and uniform, and accelerated the remodeling of collagen. Conclusions The results suggest that KBG has healing effects on skin wound in mice by anti-inflammatory and antioxidant activity.

Keywords

References

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