한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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PET분해효소(PETase) 과발현 전세포 촉매의 해양미세플라스틱 생분해 활성 연구

Biodegradation of marine microplastics by the whole-cell catalyst overexpressing recombinant PETase

  • 김현지 (부경대학교 화학공학과) ;
  • 박종하 (부경대학교 화학공학과) ;
  • 박애란 (부경대학교 화학공학과) ;
  • 이대희 (강릉원주대학교 해양식품공학과) ;
  • 전준호 (부경대학교 소방공학과) ;
  • 권혁택 (부경대학교 화학공학과) ;
  • 임성인 (부경대학교 화학공학과)
  • Hyunji, Kim (Pukyong National University/Department of Chemical Engineering) ;
  • Jong-Ha, Park (Pukyong National University/Department of Chemical Engineering) ;
  • Ae-Ran, Park (Pukyong National University/Department of Chemical Engineering) ;
  • Dae-Hee, Lee (Gangneung-Wonju National University/Department of Marine Food Science and Technology) ;
  • Joonho, Jeon (Pukyong National University/Department of Fire Protection Engineering) ;
  • Hyuk Taek, Kwon (Pukyong National University/Department of Chemical Engineering) ;
  • Sung In, Lim (Pukyong National University/Department of Chemical Engineering)
  • 투고 : 2022.11.27
  • 심사 : 2022.12.23
  • 발행 : 2022.12.30
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초록

The increased production and consumption of polyethylene terephthalate (PET)-based products over the past several decades has resulted in the discharge of countless tons of PET waste into the marine environment. PET microparticles resulting from the physical erosion of general PET wastes end up in the ocean and pose a threat to the marine biosphere and human health, necessitating the development of new technologies for recycling and upcycling. Notably, enzyme-mediated PET degradation is an appealing option due to its eco-friendly and energy-saving characteristics. PETase, a PET-hydrolyzing enzyme originating from Ideonella sakaiensis, is one of the most thoroughly researched biological catalysts. However, the industrial application of PETase-mediated PET recycling is limited due to the low stability and poor reusability of the enzyme. Here we developed the whole-cell catalyst (WCC) in which functional PETase is attached to the outer membrane of Escherichia coli. Immunoassays are used to identify the surface-expressed PETase, and we demonstrated that the WCC degraded PET microparticles most efficiently at 30℃ and pH 9 without agitation. Furthermore, the WCC increased the PET-degrading activity in a concentration-dependent manner, surpassing the limited activity of soluble PETase above 100 nM. Finally, we demonstrated that the WCC could be recycled up to three times.

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과제정보

이 연구는 2022년 부경대학교 국립대학육성사업지원비와 2020년도 강릉원주대학교 학술연구조성비 지원에 의하여 수행되었음.

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