Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Extracts and Enzymatic Hydrolysates Derived from Sea Cucumber Stichopus japonicas Ameliorate Hepatic Injury in BisphenolA-treated Mice

비스페놀A 유도 간 손상 마우스에서 해삼(Stichopus japonicas) 추출물 및 가수분해물의 간 기능 개선 효과

  • 김세정 (금산인삼약초산업진흥원) ;
  • 조윤호 (금산인삼약초산업진흥원) ;
  • 박비오 (금산인삼약초산업진흥원) ;
  • 유대석 (금산인삼약초산업진흥원) ;
  • 김두호 (휴온스푸디언스 기업부설연구소) ;
  • 김민정 (휴온스푸디언스 기업부설연구소) ;
  • 곽연길 (휴온스푸디언스 기업부설연구소) ;
  • 김진성 (금산인삼약초산업진흥원)
  • Received : 2022.04.29
  • Accepted : 2022.07.06
  • Published : 2022.12.30
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Abstract

This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.

Keywords

Acknowledgement

본 연구는 충청남도, 서천군의 해양바이오 전략소재 및 상품화 공정개발사업에서 재정적인 지원을 받아 수행되었습니다.

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