한국버섯학회지 (Journal of Mushroom)

  • 제20권4호
  • /
  • Pages.263-269
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  • 2022
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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한입버섯 균사체 특성과 배양 적정 조건

Cryptoporus volvatus (Peck) Shear: Mycelial characteristics under different culture conditions

  • 신경화 (국립공주대학교 식물자원학과) ;
  • 박태민 (국립공주대학교 식물자원학과) ;
  • 민경구 (국립공주대학교 식물자원학과) ;
  • 석순자 (단국대학교 미생물학과) ;
  • 장명준 (국립공주대학교 식물자원학과)
  • Kyung-Hwa, Shin (Department of Plant Resources, Kongju National University) ;
  • Tae-Min, Park (Department of Plant Resources, Kongju National University) ;
  • Kyung-Gu, Min (Department of Plant Resources, Kongju National University) ;
  • Soon-Ja, Seok (Department of Microbiology, Dankook University) ;
  • Myung-Jun, Jang (Department of Plant Resources, Kongju National University)
  • 투고 : 2022.09.06
  • 심사 : 2022.11.25
  • 발행 : 2022.12.31
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초록

본 실험은 국내 자생하는 한입버섯 균에 대한 특성과 적정 배양 조건을 구명하고 인공재배의 기초적인 자료 활용을 위해 균사체의 특성과 적정 배양 조건에 관한 연구를 수행하였다. 한입버섯 KACC52303 균주의 형태 특성은 불규칙형과 굴곡형, 솜털 또는 면과 유사한 질감의 형태를 보였고 미세구조는 후막포자 형성 전 단계로 보였으며 클램프는 보이지 않았다. 적정 배양온도는 중·고온인 25~30℃로 조사되었으며, pH는 약산성 4부터 중성 7까지 범위가 넓게 확인되었다. 적정 영양원에서 탄소원 dextrine 2.5%, 유기질소원 yeast extract 0.1%의 혼합배지에서 균사생장이 양호하였다. 아미노산은 leucine, 유기산은 acetic acid, gluconic acid, 무기염류는 KCl, KH2PO4에서 가장 빠른 균사생장을 보였다. 칼럼 시험은 소나무 80%, 미강 10%, 콘코브 10% 톱밥의 혼합 배지에서 가장 양호한 균사생장을 보였다.

This study investigated the culture characteristics of Cryptoporus volvatus, whichis grow naturally in Korea, to determine the suitable environmental conditions for its cultivation. The physiological characteristics of the mycelia were assessed according to the cultivation conditions, to determine the optimal conditions for artificial cultivation. The visual characteristics of the hyphae of Cryptoporus volvatus KACC52303 included an irregular and uneven surface and a fuzzy or cotton-like texture. Under the microscope, its microstructure showed pre-chlamydospore formation, but no clamps were seen. The appropriate culture temperature was found to be a medium/high temperature of approximately 25-30℃, and the optimal pH was found to have a wide range from weakly acidic (pH 4) to neutral (pH 7). In the optimal nutrient source experiment, hyphal growth was shown to be fair in a mixed medium with 2.5% dextrin as the carbon source and 0.1% yeast extract as the organic nitrogen source. Among the various amino acids, organic acids, and inorganic salts tested, the fastest hyphal growth was observed in the presence of leucine, acetic acid or gluconic acid, and KCl or KH2PO4, respectively. The column test showed that the best mycelial growth occurred in a mixed medium of 80% pine sawdust, 10% rice bran, and 10% corncob sawdust.

키워드

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