Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Effects of Ginsenoside Rb1 Loaded Films on Oral Wound Healing

Ginsenoside Rb1함유 필름의 구강 내 창상 회복 촉진 효과

  • Jeong Hyun, Lee (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Seung Hwan, Park (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Asiri Naif, Mohammed (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Myoung-Han, Lee (Jinwoo Bio Co., Ltd.) ;
  • Dong-Keon, Kweon (Jinwoo Bio Co., Ltd.) ;
  • Yongkwon, Chae (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Koeun, Lee (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Misun, Kim (Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University) ;
  • Hyoseol, Lee (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Sungchul, Choi (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital) ;
  • Ok Hyung, Nam (Department of Pediatric Dentistry, Kyung Hee University Dental Hospital)
  • 이정현 (경희대학교 치과병원 소아치과) ;
  • 박승환 (경희대학교 치과병원 소아치과) ;
  • ;
  • 이명한 ((주)진우바이오) ;
  • 권동건 ((주)진우바이오) ;
  • 채용권 (경희대학교 치과병원 소아치과) ;
  • 이고은 (경희대학교 치과병원 소아치과) ;
  • 김미선 (경희대학교 치과대학 소아치과학교실) ;
  • 이효설 (경희대학교 치과병원 소아치과) ;
  • 최성철 (경희대학교 치과병원 소아치과) ;
  • 남옥형 (경희대학교 치과병원 소아치과)
  • Received : 2022.05.10
  • Accepted : 2022.07.09
  • Published : 2022.08.31
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Abstract

This study aimed to evaluate the effects of two ginsenoside Rb1 (G-Rb1) loaded films on oral wound healing. Two types of G-Rb1 films, G-Rb1 loaded carboxymethyl cellulose (GCMC) film and G-Rb1 loaded hyaluronic acid (GHA) film, were developed. A total of 36 Sprague-Dawley rats were divided into 3 groups: control, GCMC, and GHA. After wound formation on midpalate, the control group was left without treatment, whereas the experimental groups had films attached. The specimen was analyzed clinically and histologically after 7 and 21 days. For clinical analysis, the area of incompletely re-epithelialized wound was measured. For histological analysis, the distance between the margins of the wound (soft tissue gap) was measured and the percentage of the collagen-stained area on the specimen was calculated. In clinical and soft tissue gap analysis, the GCMC group presented improved healing compared to the GHA group and the control at day 7 (p < 0.05). And, both GCMC (9.74 ± 10.12%) and GHA groups (19.50 ± 14.47%) presented greater collagen-positive pixels compared to control (0.89 ± 1.60%) at day 7 (p < 0.05). However, there were no differences in these parameters among the groups on day 21. Therefore, G-Rb1 loaded films improved oral wound healing.

이 연구의 목적은 Ginsenoside Rb1이 함유된 2가지 원형 필름의 구강 내 창상 치유에 대한 효과를 평가하는 것이다. 총 36마리의 백서를 각각 대조군, GCMC군, GHA군의 세 가지 군으로 나누어 연구를 진행하였다. 실험 동물의 정중구개부에 창상을 형성한 후, 대조군은 개방 창상으로 남겨두고, 실험군에는 각각의 필름을 적용하였다. 시편은 7일차와 21일차에 임상적, 조직학적 분석을 시행하였다. 임상적 분석을 위하여 재상피화가 일어나지 않은 창상의 면적을 계산하였고, 조직학적 분석을 위하여 스캔된 조직 표본 상에서 창상의 변연 사이 거리(Soft tissue gap)와 Collagen이 염색된 면적의 백분율을 측정하였다. 7일 희생군의 임상적 분석과 조직학적 분석 중 창상의 변연 사이 거리 모두에서 GCMC 군이 대조군과 GHA 군에 비해 높은 치유율을 보였다(p < 0.05). 7일 희생군의 GCMC 군과 GHA 군 모두에서 대조군에 비해 콜라겐 합성이 많이 발생하였다(p < 0.05). 하지만, 21일 희생군에서는 각 군간에 임상적 및 조직학적 분석 모두에서 유의미한 차이가 나타나지 않았다. 이 연구를 통해 Ginsenoside Rb1 함유 필름이 구강 내 창상 치유를 향상시킴을 확인하였다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1C1C1006937) and by the fund from Korean Academy of Pediatric Dentistry (2020).

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