Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Effect of Potassium Iodide on Erythrosine-Mediated Photodynamic Therapy on Streptococcus Mutans Biofilms

Streptococcus mutans 바이오필름에 대한 에리스로신 매개 광역동 치료 시 potassium iodide의 효과

  • Yongsoon, Kim (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Howon, Park (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Juhyun, Lee (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Haeni, Kim (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Siyoung, Lee (Department of Microbiology and Immunology, College of Dentistry, Gangneung-Wonju National University)
  • 김용순 (강릉원주대학교 치과대학 소아.청소년치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아.청소년치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉원주대학교 치과대학 소아.청소년치과학교실 및 구강과학연구소) ;
  • 김해니 (강릉원주대학교 치과대학 소아.청소년치과학교실 및 구강과학연구소) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학 및 면역학교실)
  • Received : 2022.07.04
  • Accepted : 2022.07.29
  • Published : 2022.08.31
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Abstract

The aim of this in-vitro study is to evaluate the effect of potassium iodide (KI) on erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans biofilms. S. mutans ATCC 25175 was cultured to form a biofilm on a hydroxyapatite disk. After diluting erythrosine to 20 μM and KI to 10, 50, and 100 mM, respectively, PDT was performed. The number of surviving bacteria was calculated as colony forming units (CFU)/mL and the statistical significance of the difference between groups was confirmed by Bonferroni post-hoc analysis. Cell viability was visually evaluated using confocal laser scanning microscopy (CLSM). As a result of the experiment, a significant decrease (p < 0.05) in CFU was observed in the experimental groups in which PDT was performed after applying KI regardless of the concentration of KI. In addition, a significant reduction (p < 0.05) in CFU was observed in the experimental group to which 100 mM KI was applied compared to 10 mM KI. The same results were confirmed when observing CLSM. KI significantly improved the efficacy of erythrosine-mediated PDT on S. mutans biofilms at all concentrations. This may compensate for the low sensitivity of PDT to biofilm-state bacteria strains, but it is necessary to establish an optimal clinical protocol through further research.

이 연구는 Streptococcus mutans 바이오필름에 대한 에리스로신 매개 광역동 치료에서 potassium iodide (KI)의 효과를 평가하는 In vitro 실험이다. S. mutans ATCC 25175를 배양하여 hydroxyapatite disk에 바이오필름을 형성하였다. 광감각제인 에리스로신을 20 μM, KI를 각각 10, 50, 100 mM로 희석하여 적용한 뒤 광역동 치료를 시행하였다. 생존한 세균의 수는 colony forming units (CFU)/mL로 산정하였으며 Bonferroni 사후 분석을 통해 그룹 간 차이의 유의성을 확인하였다. Confocal laser scanning microscopy (CLSM)을 이용하여 세포 생존율을 시각적으로 평가하였다. 실험 결과 KI를 적용한 후 광역동 치료를 시행한 실험군에서 KI의 농도의 관계 없이 현저한 CFU의 감소가 관찰되었다(p < 0.05). 또한 10 mM KI에 비해 100 mM KI를 적용한 실험군에서 유의한 CFU의 감소가 관찰되었다(p < 0.05). CLSM 관찰 시에도 동일한 결과를 확인하였다. KI는 모든 농도에서 S. mutans 바이오필름에 대한 에리스로신 매개 광역동 치료의 효과를 유의하게 향상시켰다. 이는 바이오필름 상태의 균주에 대한 광역동 치료의 낮은 감수성을 보완할 수 있지만, 추가적인 연구를 통한 최적의 임상 프로토콜 확립이 필요하다.

Keywords

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