Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Preparation and Characterization of Cellulose Nanofibrils from Lignocellulose Using a Deep Eutectic Solvent Followed by Enzymatic Treatment

  • Eun-Ah ,LEE (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Song-Yi, HAN (Institute of Forest Science, Kangwon National University) ;
  • Gu-Joong, KWON (Institute of Forest Science, Kangwon National University) ;
  • Jeong-Ki, KIM (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Rajkumar, BANDI (Institute of Forest Science, Kangwon National University) ;
  • Ramakrishna, DADIGALA (Institute of Forest Science, Kangwon National University) ;
  • Ji-Soo, PARK (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Chan-Woo, PARK (Institute of Forest Science, Kangwon National University) ;
  • Seung-Hwan, LEE (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University)
  • Received : 2022.08.04
  • Accepted : 2022.11.11
  • Published : 2022.11.25
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Abstract

Lignocellulose nanofibrils (LCNFs) were prepared using a two-step deep eutectic solvent (DES) and enzymatic pretreatment followed by mechanical defibrillation, and we examined the effects of enzymatic pretreatment conditions on different characteristics of the LCNFs thus obtained. The LCNFs yielded using the two-step DES pretreatment (Enz-LCNF) exhibited a well-defibrillated entangled web-like structure with an average fiber diameter ranging from 15.7 to 20.4 nm. Furthermore, we found that the average diameter and filtration time of the Enz-LCNFs decreased with an increase in enzyme concentration and enzymatic treatment time, whereas we detected a concomitant reduction in the tensile strength of the Enz-LCNF sheets. The Enz-LCNFs were characterized by a typical cellulose I structure, thereby indicating that the enzymatic treatment causes very little damage to the crystalline form.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (No. 2018R1A6A1A03025582, 2020R1I1A1A01073601).

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