The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Development of Simultaneous Analytical Method for Five Lignans in Edible Seeds

종자류 식품에 함유된 5종 리그난의 동시 분석법 개발

  • Yoonjeong, Kim (Dept. of Food Science & Biotechnology, Kyungsung University) ;
  • Jiye, Pyeon (Dept. of Food Science & Biotechnology, Kyungsung University) ;
  • In-hwan, Baek (College of Pharmacy, Kyungsung University) ;
  • Younghwa, Kim (Dept. of Food Science & Biotechnology, Kyungsung University)
  • 김윤정 (경성대학교 식품생명공학과) ;
  • 편지예 (경성대학교 식품생명공학과) ;
  • 백인환 (경성대학교 약학과 ) ;
  • 김영화 (경성대학교 식품생명공학과)
  • Received : 2022.11.25
  • Accepted : 2022.12.13
  • Published : 2022.12.31
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Abstract

There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 ㎍/100 g and the limits of quantification were 0.114~1.532 ㎍/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 ㎍/100 g, 78.965 ㎍/100 g, and 165.521 ㎍/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

Keywords

Acknowledgement

이 논문은 2022년도 농촌진흥청 연구사업(세부과제번호: PJ0170702022) 및 교육부의 재원으로 한국기초과학지원연구원 국가연구시설장비진흥센터의 지원(201900980004)을 받아 수행되었고, 부산광역시 및 (재)부산인재평생교육진흥원의 BB21플러스 사업에 의하여 지원되었습니다.

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