Parasites, Hosts and Diseases

  • 제59권5호
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  • Pages.439-445
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  • 2021
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Expression of Chicken NK-Lysin and Its Role in Chicken Coccidiosis Induced by Eimeria necatrix

  • Kim, Woo Hyun (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University) ;
  • Min, Wongi (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University) ;
  • Park, Kwang Il (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University) ;
  • Lillehoj, Hyun S. (Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, U.S. Department of Agriculture) ;
  • Fernandez-Colorado, Cherry P. (Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos) ;
  • Flores, Rochelle A. (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University) ;
  • Cammayo, Paula Leona T. (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University) ;
  • Nguyen, Binh Thanh (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
  • 투고 : 2021.08.10
  • 심사 : 2021.10.11
  • 발행 : 2021.10.31
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초록

Coccidiosis in chickens is an intestinal parasitic disease caused by protozoan parasites named Eimeria spp. In some Eimeria infections, intestinal lymphocytes are known to highly express chicken NK-lysin (cNK-lysin), an antimicrobial peptide with anticoccidial activity. Therefore, this study aims to investigate the expression of cNK-lysin in E. necatrix-infected chickens and its role in E. necatrix infection. The expression of cNK-lysin transcript was significantly increased in E. necatrix sporozoites-treated lymphocytes. In E. necatrix infection, cNK-lysin transcript was induced in intestinal lymphocytes but not in the spleen. The recombinant cNK-lysin exhibited anticoccidial activity against E. necatrix sporozoites as well as immunomodulatory activity on macrophages by inducing proinflammatory cytokines. These results indicated that E. necatrix infection induces high local expression of cNK-lysin and the secreted cNK-lysin helps protect coccidiosis.

키워드

과제정보

This work was supported by IPET through the Animal Disease Management Technology Development Program, funded by MAFRA (320062-2).

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