Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Trends in the development of discriminating between Angelica L. species using advanced DNA barcoding techniques

진보된 DNA barcoding 기술을 이용한 당귀(Angelica)속 식물의 기원 판별 기술에 관한 연구 동향

  • Lee, Shin-Woo (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Shin, Yong-Wook (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
  • 이신우 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 신용욱 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 김윤희 (경상국립대학교 사범대학 생물교육과 (농업생명과학연구원))
  • Received : 2021.08.18
  • Accepted : 2021.08.31
  • Published : 2021.09.30
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Abstract

We reviewed current research trends for discriminating between species of the Angelica genus, a group of important medicinal plants registered in South Korea, China, and Japan. Since the registered species for medicinal purposes differ by country, they are often adulterated as well as mixed in commercial markets. Several DNA technologies have been applied to distinguish between species. However, one of the restrictions is insufficient single-nucleotide polymorphisms (SNPs) within the target DNA fragments; in particular, among closely-related species. Recently, amplification refractory mutation system (ARMS)-PCR and highresolution melting (HRM) curve analysis techniques have been developed to solve such a problem. We applied both technologies, and found they were able to discriminate several lines of Angelica genus, including A. gigas Nakai, A. gigas Jiri, A. sinensis, A. acutiloba Kitag, and Levisticum officinale. Furthermore, although the ITS region differs only by one SNP between A. gigas Nakai and A. gigas Jiri, both HRM and ARMS-PCR techniques were powerful enough to discriminate between them. Since both A. gigas Nakai and A. gigas Jiri are native species to South Korea and are very closely related, they are difficult to discriminate by their morphological characteristics. For practical applications of these technologies, further research is necessary with various materials, such as dried or processed materials (jam, jelly, juice, medicinal decoctions, etc.) in commercial markets.

본 리뷰에서는 우리나라, 중국, 일본 등에서 각각 그 기원식물을 달리하는 당귀 속 식물 계통의 기원 계통을 판별하기 위한 DNA barcoding 기술의 발전현황에 관하여 조사하였다. 약용작물들에 대한 종의 기원을 판별하기 위하여 단일염기다형성을 이용한 DNA 바코드의 개발에 관한 연구가 활발하게 진행되어왔다. 그러나 가까운 근연종간에는 단일염기다형성을 보이는 염기의 수가 많지 않아 어려움이 있었다. 이러한 문제점을 해결하기 위하여 ARMS-PCR 및 HRM curve 패턴 비교 분석기술 등이 개발되었다. 이들 기술을 적용하여 국내 자생종 및 국외에서 수집된 당귀 계통들에 대하여 이들의 기원을 판별 할 수 있는 조건이 확립되었다. 특히 단하나의 단일염기다형성을 보이는 국내 자생종인 참당귀와 세발당귀의 판별이 가능하여 향후 현장에 적용이 가능한 실용화 연구가 필요한 것으로 조사되었다. 그러나 이들 연구결과는 그 기원이 확인된 계통의 시료들을 대상으로 분리한 순수 DNA를 대상으로 조사한 결과로, 현장에서 실용화하기에는 아직 보다 많은 연구가 필요하다. 실제로 일정한 비율로 혼합한 계통들을 대상으로 분리한 DNA를 대상으로 한 후속 연구가 필요하다. 또한, 수확 후 가공 및 처리 방법에 따른 시료들에 대한 후속 연구도 필요하다. 당귀와 같은 약용작물은 건조한 시료, 다양한 가공제품(잼, 잴리, 쥬스 등), 약탕(탕재) 등으로 유통이 되기 때문에 이들에 대한 시료별 적용 가능성에 대한 연구도 필요한 것으로 조사되었다.

Keywords

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