한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제40권2호
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  • Pages.134-141
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  • 2021
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
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축산물 중 살균제 Chinomethionat의 개별 잔류분석법 확립

Development of Analytical methods for Chinomethionat in Livestock Products

  • 양승현 (원광대학교 농식품융합대학 생물환경화학과) ;
  • 김정한 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 최훈 (원광대학교 농식품융합대학 생물환경화학과)
  • Yang, Seung-Hyun (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University)
  • 투고 : 2021.06.04
  • 심사 : 2021.06.23
  • 발행 : 2021.06.30
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초록

본 연구는 축산물 중 퀴녹사린계 살균제 chinomethionat의 잔류분석법을 확립하였다. 소고기, 돼지고기, 닭고기, 우유, 계란을 대표 시료로 선정하고 GC-ECD를 이용한 chinomethionat 단성분 정량 시험법을 개발하였다. 축산물 중 chinomethionat 잔류물을 acetone/dichloromethane (9/1, v/v)로 추출하고 추출첨가제 MgSO4 및 NaCl을 첨가한 후, dichloromethane로 분배하고 Florisil 흡착제로 정제하였다. 축산물 중 chinomethionat 정량한계는 0.02 mg/kg으로 결정되었으며, MLOQ 수준의 회수율은 84.8-100.9%, MLOQ 10배 수준에서는 85.7-103.0%의 우수한 회수율을 보였으며, 분석오차는 최대 5.2%로 재현성 역시 양호하였다. 본 연구에서 확립한 chinomethionat의 잔류분석법은 국내·외 축산물의 잔류농약 검사 및 분석에 적용 가능할 것으로 기대된다.

BACKGROUND: The analytical method was established for determination of fungicide chinomethionat in several animal commodities using gas chromatography (GC) coupled with electron capture detector (ECD). METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining chinomethonat in various livestock products including beef, pork, chicken, milk and egg. Chinomethionat residual was extracted using acetone/dichloromethane(9/1, v/v) with magnesium sulfate and sodium chloride (salting outassociated liquid-liquid extraction). The extract was diluted by direct partitioning into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was finally purified with optimized silica gel 10 g. CONCLUSION: The method limit of quantitation (MLOQ) was 0.02 mg/kg, which was in accordance with the maximum residue level (MRL) of chinomathionate as 0.05 mg/kg in livestock product. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (84.8~103.0%). Reproducibilities were obtained (Coefficient of variation <5.2%), and the linearity of calibration curves were reasonable (r2>0.995) in the range of 0.01-0.2 ㎍/mL. This established analytical method was fully validated and could be useful for quantification of chinomathionat in animal commodities as official analytical method.

키워드

과제정보

This research was supported by the Ministry of Food and Drug Safety, Republic of Korea (grant number: 16162MFDS586).

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