Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Development of Analytical methods for Chinomethionat in Livestock Products

축산물 중 살균제 Chinomethionat의 개별 잔류분석법 확립

  • Yang, Seung-Hyun (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Food Sciences, Wonkwang University)
  • 양승현 (원광대학교 농식품융합대학 생물환경화학과) ;
  • 김정한 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 최훈 (원광대학교 농식품융합대학 생물환경화학과)
  • Received : 2021.06.04
  • Accepted : 2021.06.23
  • Published : 2021.06.30
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Abstract

BACKGROUND: The analytical method was established for determination of fungicide chinomethionat in several animal commodities using gas chromatography (GC) coupled with electron capture detector (ECD). METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining chinomethonat in various livestock products including beef, pork, chicken, milk and egg. Chinomethionat residual was extracted using acetone/dichloromethane(9/1, v/v) with magnesium sulfate and sodium chloride (salting outassociated liquid-liquid extraction). The extract was diluted by direct partitioning into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was finally purified with optimized silica gel 10 g. CONCLUSION: The method limit of quantitation (MLOQ) was 0.02 mg/kg, which was in accordance with the maximum residue level (MRL) of chinomathionate as 0.05 mg/kg in livestock product. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (84.8~103.0%). Reproducibilities were obtained (Coefficient of variation <5.2%), and the linearity of calibration curves were reasonable (r2>0.995) in the range of 0.01-0.2 ㎍/mL. This established analytical method was fully validated and could be useful for quantification of chinomathionat in animal commodities as official analytical method.

본 연구는 축산물 중 퀴녹사린계 살균제 chinomethionat의 잔류분석법을 확립하였다. 소고기, 돼지고기, 닭고기, 우유, 계란을 대표 시료로 선정하고 GC-ECD를 이용한 chinomethionat 단성분 정량 시험법을 개발하였다. 축산물 중 chinomethionat 잔류물을 acetone/dichloromethane (9/1, v/v)로 추출하고 추출첨가제 MgSO4 및 NaCl을 첨가한 후, dichloromethane로 분배하고 Florisil 흡착제로 정제하였다. 축산물 중 chinomethionat 정량한계는 0.02 mg/kg으로 결정되었으며, MLOQ 수준의 회수율은 84.8-100.9%, MLOQ 10배 수준에서는 85.7-103.0%의 우수한 회수율을 보였으며, 분석오차는 최대 5.2%로 재현성 역시 양호하였다. 본 연구에서 확립한 chinomethionat의 잔류분석법은 국내·외 축산물의 잔류농약 검사 및 분석에 적용 가능할 것으로 기대된다.

Keywords

Acknowledgement

This research was supported by the Ministry of Food and Drug Safety, Republic of Korea (grant number: 16162MFDS586).

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