The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Detection of RNA Mycoviruses in Wild Strains of Lentinula edodes in Korea

  • Kim, Eunjin (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science) ;
  • Park, Mi-Jeong (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science) ;
  • Jang, Yeongseon (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science) ;
  • Ryoo, Rhim (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Forest Microbiology Division, Forest Bioresources Department, National Institute of Forest Science)
  • Received : 2021.06.12
  • Accepted : 2021.08.24
  • Published : 2021.09.30
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Abstract

In general, mycoviruses remain latent and rarely cause visible symptoms in fungal hosts; however, some viral infections have demonstrated abnormal mycelial growth and fruiting body development in commercial macrofungi, including Lentinula edodes. Compared to other cultivated mushrooms, L. edodes is more vulnerable to viral infections as it is still widely cultivated under near-natural conditions. In this study, we investigated whether Korean wild strains of L. edodes were infected by RNA mycoviruses that have previously been reported in other parts of the world (LeSV, LePV1, LeV-HKB, LeNSRV1, and LeNSRV2). Using specific primer sets that target the RNA-dependent RNA polymerase genes of each of the RNA mycovirus, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect viral infection. Viral infection was detected in about 90% of the 112 wild strains that were collected in Korea between 1983 and 2020. Moreover, multiple infections with RNA mycoviruses were detected in strains that had normal fruiting bodies. This work contributes to our understanding of the distribution of RNA mycoviruses in Korea and the impact of multiple viral infections in a single strain of L. edodes.

Keywords

Acknowledgement

This study was supported by a grant from the Golden Seed Project of 'Breeding of new strains of shiitake for cultivar protection and substitution of import (213007-05-5-SBH10)' of the National Institute of Forest Science, Republic of Korea.

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