Journal of Microbiology and Biotechnology

  • 제31권9호
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  • Pages.1288-1294
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  • 2021
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Synergistic Antibacterial Activity of an Active Compound Derived from Sedum takesimense against Methicillin-Resistant Staphylococcus aureus and Its Clinical Isolates

  • Jeong, Eun-Tak (DYNE SOZE Co., Ltd.) ;
  • Park, Seul-Ki (Institute of Food Science, Pukyong National University) ;
  • Jo, Du-Min (Department of Food Science and Technology, Pukyong National University) ;
  • Khan, Fazlurrahman (Research Center for Marine Integrated Bionics Technology, Pukyong National University) ;
  • Choi, Tae Ho (DYNE SOZE Co., Ltd.) ;
  • Yoon, Tae-Mi (DYNE SOZE Co., Ltd.) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • 투고 : 2021.05.12
  • 심사 : 2021.07.19
  • 발행 : 2021.09.28
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초록

There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-O-galloyl-glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.

키워드

과제정보

This research was financially supported by the Ministry of Small and Medium-sized Enterprises (SMEs) and Startups (MSS), Korea, under the "Regional Specialized Industry Development Plus Program (R&D, S2874391)" supervised by the Korea Institute for Advancement of Technology (KIAT). This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2019R1A2C1087156 and 2021R1A6A1A03039211).

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