DOI QR코드

DOI QR Code

Detection of AluI Endonuclease Activity by Using Double Stranded DNA-Templated Copper Nanoclusters

  • 투고 : 2021.03.01
  • 심사 : 2021.05.26
  • 발행 : 2021.09.28

초록

Restriction endonucleases play an important role in molecular cloning, clinical diagnosis, and pharmacological drug studies. In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect AluI endonuclease activity due to their high fluorescence emission and rapid synthesis of DNA-CuNCs under ambient conditions. Results showed that AluI activity was detected in a highly sensitive manner at low concentrations of AluI endonuclease by the fluorescence intensity of DNA-CuNCs. Additionally, its inhibition was monitored in the presence of daidzein under optimal conditions.

키워드

참고문헌

  1. Brueckner B, Lyko F. 2006. DNA methyltransferase inhibitors: old and new drugs for an epigenetic cancer therapy. Trends Pharmacol. Sci. 25: 551-554. https://doi.org/10.1016/j.tips.2004.09.004
  2. Shames DS, Minna JD, Gazdar AF. 2007. DNA methylation in health, disease, cander. Curr. Mol. Med. 7: 85-102. https://doi.org/10.2174/156652407779940413
  3. Norberg P, Bergstrom T, Liljeqvist JA. 2006. Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes. J. Clin. Microbiol. 44: 4511-4514. https://doi.org/10.1128/JCM.00421-06
  4. Xu X, Han MS, Mirkin CA. 2007. A gold-nanoparticle-based realtime colorimetric screening method for endonuclease activity and inhibition. Angew. Chem. Int. Ed. 46: 3468-3470. https://doi.org/10.1002/anie.200605249
  5. Harford SE, Goodall AJ. 1988. Mode of DNA cleavage by the EcoRV restriction endonuclease. Biochemistry 27: 1771-1777. https://doi.org/10.1021/bi00405a058
  6. Jeltsch A, Fritz A, Alves A, Wolfes H, Pingoud A. 1993. A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases. Anal. Biochem. 213: 234-240. https://doi.org/10.1006/abio.1993.1415
  7. Sharp PA, Sugden B, Sambrock J. 1973. Detection of two restriction endonuclease activities in Haemophilus Parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry 12: 3055-3063. https://doi.org/10.1021/bi00740a018
  8. Song G, Chen C, Ren J, Qu X. 2009. A simple, universal colorimetric assay for endonuclease/methyltransferase activity and inhibition based on an enzyme-responsive nanoparticle system. ACS Nano 3: 1183-1189. https://doi.org/10.1021/nn800768z
  9. Zhao H, Dong J, Zhou F, Li B. 2017. One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators. Sens. Actuators B Chem. 238: 828-833. https://doi.org/10.1016/j.snb.2016.07.083
  10. Feng X, Duan X, Liu L, Feng F, Wang S, Li Y, et al. 2009. Fluorescence logic-signal-based multiplex detection of nucleases with the assembly of a cationic conjugated polymer and branched DNA. Angew. Chem. Int. Ed. 48: 5316-5321. https://doi.org/10.1002/anie.200901555
  11. Deng J, Jin Y, Wang L, Chen G, Zhang C. 2012. Sensitive detection of endonuclease activity and inhibition using gold nanorods. Biosens. Bioelectron. 34: 144-150. https://doi.org/10.1016/j.bios.2012.01.034
  12. Lee C, Gang J. 2018. A Label-free detection of NdeI endonuclease activity by using DNA-templated silver nanoclusters. J. Nanosci. Nanotechnol. 18: 6339-6342. https://doi.org/10.1166/jnn.2018.15657
  13. Huang Y, Zhao S, Chen ZF, Liu YC, Liang H. 2011. Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization. Chem. Commun. 47: 4763-4765. https://doi.org/10.1039/c1cc10325c
  14. Li W, Liu Z, Lin H, Nie Z, Chen J, Xu X, et al. 2010. Label-free colorimetric assay for methyltransferase activity based on a novel methylation-responsive DNAzyme strategy. Anal. Chem. 82: 1935-1941. https://doi.org/10.1021/ac902670c
  15. Zhao Y, Chen F, Wu Y, Dong Y, Fan C. 2013. Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signal amplification. Biosens. Bioelectron. 42: 56-61. https://doi.org/10.1016/j.bios.2012.10.022
  16. Bi S, Zhao T, Luo B, Zhu JJ. 2013. Hybridization chain reaction-based branched rolling circle amplification for chemiluminescence detection of DNA methylation. Chem. Commun. 49: 6906-6908. https://doi.org/10.1039/c3cc43353f
  17. Cao A, Zhang CY. 2012. Sensitive and Label-free DNA methylation detection by ligation-mediated hyperbranched rolling circle amplification. Anal. Chem. 84: 6199-6205. https://doi.org/10.1021/ac301186j
  18. Jia H, Li Z, Liu C, Cheng Y. 2010. Ultrasensitive detection of microRNAs by exponential isothermal amplification. Angew. Chem. Int. Ed. 49: 5498-5501. https://doi.org/10.1002/anie.201001375
  19. Zhou Z, Du Y, Zhang L, Dong S. 2012. A Label-free, G-quadruplex DNAzyme-based fluorescent probe for signal-amplified DNA detection and turn-on assay of endonuclease. Biosens. Bioelectron. 34: 100-105. https://doi.org/10.1016/j.bios.2012.01.024
  20. Jiang XQ, Guo SM, Zhou M, Ye BC. 2014. DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition. Analyst 139: 5682-5685. https://doi.org/10.1039/c4an01373e
  21. Zhao H, Dong J, Zho F, Li B. 2017. One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators. Sens. Acuators B Chem. 238: 828-833. https://doi.org/10.1016/j.snb.2016.07.083
  22. Rotaru A, Dutta S, Jentzsch E, Gothelf K, Mokhir A. 2010. Selective dsDNA-templated formation of copper nanoparticles in solution. Angew. Chem. Int. Ed. 49: 5665-5667. https://doi.org/10.1002/anie.200907256
  23. Maniotis AJ, Valyi-Nagy K, Karavitis J, Moses J, Boddipali V, Wang Y, et al. 2005. Chromatin organization measured by AluI restriction endonuclease changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton. Am. J. Pathol. 166: 1187-1203. https://doi.org/10.1016/S0002-9440(10)62338-3
  24. Sandal T, Valyi-Nagy K, Spencer VA, Folberg R, Bissell MJ, Maniotis AJ. 2007. Epigenetic reversion of breast carcinoma phenotype is accompanied by changes in DNA sequestration as measured by AluI restriction enzyme. Am. J. Pathol. 170: 1739-1749. https://doi.org/10.2353/ajpath.2007.060922