Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory and Cytotoxic Activities of Phenolic Compounds from Broussonetia kazinoki

  • Vu, Ngoc Khanh (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Le, Thi Thanh (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Woo, Mi Hee (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University) ;
  • Min, Byung Sun (College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
  • Received : 2021.07.12
  • Accepted : 2021.08.29
  • Published : 2021.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The phytochemical investigation of Broussonetia kazinoki roots led to the isolation of ten compounds, including six flavonoids (1-6), two lignans (7 and 8), and two coumarins (9 and 10) by comparing their 1H and 13C NMR spectra with reference values. To the best of our knowledge, compounds 9 and 10 were isolated from this plant for the first time. Among the ten isolates, compounds 2, 4, and 6 exhibited inhibitory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW264.7 cells with IC50 values of 11.98, 10.16, and 24.06 μM, respectively. Furthermore, compounds 2, 4, and 6 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. Pre-incubation of cells with these compounds also significantly suppressed LPS-induced COX-2 protein expression. Compounds 2, 4, and 6 also showed cytotoxic activity against HL-60 cells with IC50 values ranging between 46.43 and 94.06 μM.

Keywords

Acknowledgement

This study was supported by the research grant from Daegu Catholic University, Korea in 2021 (Grant No. DCU-20211113).

References

  1. Mosmann, T. J. Immunol. Methods 1983, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  2. Tran, H. N. K.; Cao, T. Q.; Kim, J. A.; Woo, M. H.; Min, B. S. Fitoterapia 2019, 137, 104261. https://doi.org/10.1016/j.fitote.2019.104261
  3. Evans, C. H. Agents Actions Suppl. 1995, 47, 107-116.
  4. Tabas, I.; Glass, C. K. Science 2013, 339, 166-172. https://doi.org/10.1126/science.1230720
  5. Moncada, S.; Palmer, R. M.; Higgs, E. A. Pharmacol. Rev. 1991, 43, 109-142.
  6. Maioral, M. F.; do Nascimento Bodack, C.; Stefanes, N. M.; Bigolin, A.; Mascarello, A.; Chiaradia-Delatorre, L. D.; Yunes, R. A.; Nunes, R. J.; Santos-Silva, M. C. Biochimie 2017, 140, 48-57. https://doi.org/10.1016/j.biochi.2017.06.004
  7. Ruffa, M.; Ferraro, G.; Wagner, M. L.; Calcagno, M. L.; Campos, R. H.; Cavallaro, L. J. Ethnopharmacol. 2002, 79, 335-339. https://doi.org/10.1016/S0378-8741(01)00400-7
  8. Bracci, L.; Schiavoni, G.; Sistigu, A.; Belardelli, F. Cell Death Differ. 2014, 21, 15-25. https://doi.org/10.1038/cdd.2013.67
  9. Zhang, P. C.; Wang, S.; Wu, Y.; Chen, R. Y.; Yu, D. Q. J. Nat. Prod. 2001, 64, 1206-1209. https://doi.org/10.1021/np010283o
  10. Wang, G. W.; Huang, B. K.; Qin, L. P. Phytother. Res. 2012, 26, 1-10. https://doi.org/10.1002/ptr.3575
  11. Lee, H.; Li, H.; Jeong, J. H.; Noh, M.; Ryu, J. H. Fitoterapia 2016, 112, 90-96. https://doi.org/10.1016/j.fitote.2016.05.006
  12. Jadeja, Y. S.; Kapadiya, K. M.; Jebaliya, H. J.; Shah, A. K.; Khunt, R. C. Magn. Reson. Chem. 2017, 55, 589-594. https://doi.org/10.1002/mrc.4559
  13. Encarnacion, D. R.; Nogueiras, L.; Salinas, V. H. A.; Anthoni, U.; Nielsen, P. H.; Christophersen, C. Acta Chem. Scand. 1999, 53, 375-377. https://doi.org/10.3891/acta.chem.scand.53-0375
  14. Lukacin, R.; Wellmann, F.; Britsch, L.; Martens, S.; Matern, U. Phytochemistry 2003, 62, 287-292. https://doi.org/10.1016/S0031-9422(02)00567-8
  15. Van Loo, P.; De Bruyn, A.; Budesinsky, M. Magn. Reson. Chem. 1986, 24, 879-882. https://doi.org/10.1002/mrc.1260241007
  16. Park, Y.; Moon, B. H.; Yang, H.; Lee, Y.; Lee, E.; Lim, Y. Magn. Reson. Chem. 2007, 45, 1072-1075. https://doi.org/10.1002/mrc.2063
  17. Vu, N. K.; Kim, C. S .; Ha, M. T.; Ngo, Q. M. T.; Park, S . E.; Kwon, H.; Lee, D.; Choi, J. S .; Kim, J. A.; Min, B. S . J. Agric. Food Chem. 2020, 68, 8797-8811. https://doi.org/10.1021/acs.jafc.0c02122
  18. Brenes, M.; Hidalgo, F. J.; Garcia, A.; Rios, J. J.; Garcia, P.; Zamora, R.; Garrido, A. J. Am. Oil Chem. Soc. 2000, 77, 715-720. https://doi.org/10.1007/s11746-000-0115-4
  19. Gohari, A. R.; Saeidnia, S.; Bayati-Moghadam, M.; Amin, G. Daru 2011, 19, 74-79.
  20. Lemmich, J.; Havelund, S.; Thastrup, O. Phytochemistry 1983, 22, 553-555. https://doi.org/10.1016/0031-9422(83)83044-1
  21. Xu, W.; Liu, L. Z.; Loizidou, M.; Ahmed, M.; Charles, I. G. Cell Res. 2002, 12, 311-320. https://doi.org/10.1038/sj.cr.7290133