Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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TRPV1 activation induces cell death of TM3 mouse Leydig cells

  • Kim, Eun-Jin (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Dang, Long Cao (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Nyiramana, Marie Merci (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Siregar, Adrian S. (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Woo, Min-Seok (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Chang-Woon (Department of Obstetrics and Gynecology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Kang, Dawon (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University)
  • Received : 2021.09.17
  • Accepted : 2021.09.22
  • Published : 2021.09.30
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Abstract

The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicin-induced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.

Keywords

Acknowledgement

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education(2021R1I1A3044128) and the Ministry of Science and ICT (2015R1A5A2008833).

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