Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Antibacterial and anti-obesity effects of Abeliophyllum distichum Nakai: an in vitro study

  • Song, Dong Cheol (Division of Food and Animal Science, Chungbuk National University) ;
  • Lee, Ji Hwan (Division of Food and Animal Science, Chungbuk National University) ;
  • Oh, Han Jin (Division of Food and Animal Science, Chungbuk National University) ;
  • Kim, Yong Ju (Division of Food and Animal Science, Chungbuk National University) ;
  • An, Jae Woo (Division of Food and Animal Science, Chungbuk National University) ;
  • Chang, Se Yeon (Division of Food and Animal Science, Chungbuk National University) ;
  • Go, Young Bin (Division of Food and Animal Science, Chungbuk National University) ;
  • Cho, Hyun Ah (Division of Food and Animal Science, Chungbuk National University) ;
  • Cho, Jin Ho (Division of Food and Animal Science, Chungbuk National University)
  • Received : 2021.05.31
  • Accepted : 2021.07.22
  • Published : 2021.09.01
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Abstract

Interest in research on various medicinal plants has increased globally over the last few decades, possibly due to their possible antibacterial and antioxidant activities. The present study was conducted to verify the antioxidant effects, antibacterial activity, and collagen synthesis and cell viability outcomes of adipocytes upon exposure to Abeliophyllum distichum Nakai (AdN). Antibacterial activity was measured through the Disc diffusion method to compare the growth ability of pathogenic microorganisms (E.coli, Salmonella). The absorbance was measured at 560 nm to calculate the active oxygen scavenging ability. Fibroblasts were dispensed in a 96-well plate at a density of 1 × 105 cells·well-1. The amount of procollagen was measured in each case using a procollagen type 1 C-peptide EIA KIT. The cytotoxicity of the Abeliophyllum distichum Nakai extract against animal adipocytes (Hanwoo backfat cells) was determined using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, a method that measures the conversion of MTS to Formazan by means of mitochondrial dehydrogenases. The concentrations of the samples were made to be 0.0125, 0025, 0.05, 0.1, and 0.5% and all were -completely absorbed into the disc in an incubator at 37℃ for 24 to 36 hours. For the 0.125 mg·disc-1, effects of Abeliophyllum distichum Nakai on the antioxidant effect, antibacterial activity, and cell viability of adipocytes were found. However, Abeliophyllum distichum Nakai had no effect on collagen synthesis, thus suggesting that AdN extracts may be useful for the prevention and/or treatment of obesity.

Keywords

Acknowledgement

This result was supported by "Regional Innovation Strategy (RIS)" through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE).

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