대한본초학회지 (The Korea Journal of Herbology)

  • 제36권5호
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  • Pages.81-91
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  • 2021
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

난소 적출 흰쥐 골다공증 모델에서 금은화(金銀花)가 골밀도 증가에 미치는 효과

Effect of Lonicerae Japonicae Flos on Bone Density in Ovariectomized Rat Model of Osteoporosis

  • 이성엽 (경희대학교 한의과대학 해부학교실) ;
  • 김민선 (경희대학교 한의과대학 해부학교실) ;
  • 홍수연 (경희대학교 한의과대학 해부학교실) ;
  • 김재현 (경희대학교 한의과대학 해부학교실) ;
  • 김홍식 (경희대학교 한의과대학 해부학교실) ;
  • 이충호 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실)
  • Lee, SungYub (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Minsun (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Hong, SooYeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Jae-Hyun (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Hongsik (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Chungho (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • 투고 : 2021.08.10
  • 심사 : 2021.09.25
  • 발행 : 2021.09.30
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초록

Objectives : Osteoporosis is a systemic skeletal disease that decreases bone density and increases the risk of fractures. Bisphosphonates and SERMs are mainly used to treat osteoporosis, but, long-term use increases the risk of side effects such as jaw bone necrosis and breast cancer. Therefore, it is necessary to develop a therapeutic agent for a natural product with few side effects. Water extract of Lonicerae Japonicae Flos (wLF) was mainly found to have anti-cancer and anti-inflammatory effects. However, the effect of wLF on osteoporosis has not been elucidated. Therefore, this experiment investigated the effect of wLF on osteoclasts, osteoblasts and osteoporosis models. Methods : In order to study the effect of wLF on osteoporosis, the OVX-induced rat model was used for in vivo study. After 8 weeks, we measured body weight, uterine weight, liver weight, femur weight, bone density, trabecular area and tibia ash weight. To determine the effect of wLF on osteoclast differentiation, we measured the number of TRAP-positive cells and TRAP activity. To examine the effect of wLF on the expression of osteoblast-related genes, we measured the mRNA expression of alkaline phosphatase (ALP, Alpl) and osteocalcin (OCN, Bglap2). Results : In vivo experiment, wLF inhibited the reduction of femur weight, trabecular area, bone density and tibia ash weight. In vitro experiment, wLF had no significant effect on osteoclast differentiation. However, wLF increased the mRNA expression of Alpl and Bglap2 in MC3T3-E1 cell. Conclusions : This result suggested that wLF may be used for the treatment and prevention of postmenopausal osteoporosis.

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과제정보

이 논문은 2020년도 정부 (과학기술정보통신부)의 재원으로한국연구재단의 지원을 받아 수행된 연구임 (No. 2020R1A2C1007836).

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