Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Characterization of the recombinant cellulase A from Thermotoga maritima

  • Kim, Chung Ho (Department of Food and Nutrition, Seowon University)
  • Received : 2021.07.07
  • Accepted : 2021.07.10
  • Published : 2021.09.30
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Abstract

A gene encoding thermostable cellulase A (TmCelA) was isolated from Thermotoga maritima. The open reading frame of TmCelA gene was 774 bp long which predicted to encode 257 amino acid residues with a molecular weight of 29,732 Da. To examine the biochemical properties, the TmCelA was overexpressed in E. coli BL21, and expressed protein was purified. The optimum temperature of recombinant TmCelA was 90-95 ℃, and the optimum pH of recombinant TmCelA was approximately pH 5.0. Recombinant TmCelA was stable at temperature below 90 ℃.

Keywords

Acknowledgement

This work was supported by a research grant from the Seowon University.

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