Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Optimization of the extraction procedure for quantitative analysis of saponarin and the artificial light condition for saponarin production from barley sprout

고함량 사포나린 함유 보리 어린 순 재배를 위한 식물공장내 인공광 조건 및 사포나린 추출 분석법 최적화

  • Oh, Kyeong-Yeol (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Song, Yeong Hun (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Lee, Duek-Yeong (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Lee, Tae-Geun (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Kim, Jin-Hyo (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
  • Received : 2021.06.11
  • Accepted : 2021.06.29
  • Published : 2021.09.30
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Abstract

Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m-2 s-1 of the light quantity with 8 h day-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.

본 연구에서는 보리 어린 순의 기능성 성분인 saponarin의 정량적 추출을 위한 최적의 추출 분석 조건을 methanol, ethanol, acetonitrile 및 water를 대상으로 반응표면분석법을 통해 실시하였다. 이를 통해 용매의 농도, 추출 시간에 따른 saponarin과 색소 추출율을 확인한 결과, 53.7% methanol 수용액에서 3.9 h 동안 진탕추출하는 것이 높은 saponarin 추출율을 유지하면서, 불필요한 색소의 추출을 최소화하는 최적 추출조건으로 확인되었다. 보리 어린 순의 재배에 필요한 인공광의 saponarin 생성 영향 평가를 광 주기, 광량, 광질을 달리한 조건에서 시험한 결과, saponarin 고함유 보리 어린 순의 인공광 최적 재배 조건은 8 h day-1의 광 주기에서 6500K LED와 적색보광을 통한 총 광량 220-320 μmol m-2 s-1 에서 가장 효과적임을 확인 할 수 있었고, 청색광이 saponarin 생합성에 주요 인자로 작용함을 확인할 수 있었다.

Keywords

Acknowledgement

The Basic Science Research Program supported this research through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2019R1I1A3A01042356), Republic of Korea.

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