Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative Analysis of Allantoin in Dioscorea japonica Peel Using an Amino Bonded-Phase HPLC Column

아미노 결합 정지상 HPLC 컬럼을 이용한 마 껍질의 allantoin 함량 분석

  • Lee, Mijeong (School of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Ja Min (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Kim, Hunseong (School of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hahn, Dongyup (School of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University)
  • 이미정 (경북대학교 농업생명과학대학 식품공학부) ;
  • 김자민 (경북대학교 농업과학기술연구소) ;
  • 김훈성 (경북대학교 농업생명과학대학 식품공학부) ;
  • 한동엽 (경북대학교 농업생명과학대학 식품공학부)
  • Received : 2021.07.16
  • Accepted : 2021.08.19
  • Published : 2021.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

Yam (Dioscorea japonica) is widely utilized as food and a pharmaceutical ingredient as it contains a variety of valuable constituents. Allantoin is one of the bioactive components in yam that is used in pharmaceutical and cosmetic industry. This study was conducted to analyze and compare the allantoin content of yam flesh and peel by HPLC analytical method using an amino bonded-phase column to make up for the limitations of the previous HPLC analytical methods. The allantoin contents of yam flesh and peel were 3.09±0.025 and 3.91±0.11 mg/g (dry weight), respectively. The results of this study indicated that yam peel has higher allantoin content than yam flesh, and that the discarded yam peel could be used as a source for high value-added functional materials.

마에는 다양한 기능성 성분이 함유되어 있어서 식용 및 약용으로 다양하게 이용되고 있다. 특히 allantoin은 대표적인 마의 2차 대사산물로서 의약품 및 기능성 화장품 제조에도 활용되는 고부가가치 산물이다. 본 연구에서는 아미노 결합 정지상 HPLC 컬럼을 이용하여 마의 속과 껍질의 allantoin 함량을 분석하였다. Allantoin 표준용액의 검량선의 결정계수(R2)값은 0.9999로 높은 직진성을 보였으며, 검출한계(LOD)와 정량한계(LOQ)는 각각 0.0229 mg/mL 및 0.0694 mg/mL로 설정되었다. 마 속과 껍질의 건조중량 기준 allantoin 함량은 각각 3.09±0.02 mg/g, 3.91±0.11 mg/g으로 속보다 껍질에 더 많은 allantoin이 함유되어있음을 확인하였다. 따라서 이러한 결과를 토대로 향후 농업부산물인 마껍질이 새로운 고부가가치 기능성 소재로 활용될 수 있음을 확인하였다.

Keywords

Acknowledgement

본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 유용농생명자원산업화기술개발사업의 지원을 받아 연구되었음(과제번호: 121049-02).

References

  1. Duan, Y., Kim, G.H., Joung, S.J., Kim, H.S., Nutritional evaluation of Korean yam (Dioscorea batatas DECNE). J. Korean Oil Chem. Soc., 33, 391-400 (2016). https://doi.org/10.12925/jkocs.2016.33.2.391
  2. Lee, H., Seo, D.H., Kim, H.S., Effect of starch extraction solutions on extraction and physicochemical property of Chinese yam (Dioscorea batatas) starch. Korean J. Food Sci. Technol., 50, 191-197 (2018). https://doi.org/10.9721/KJFST.2018.50.2.191
  3. Kwon, C.S., Son, I.S., Shim, J.H., Kwun, I.S., Chung, K.M.. Effects of yam on lowering cholesterol level and its mechanism. Korea J. Food & Nutr. 32, 637-643 (1999).
  4. Kwon, E.K., Choi, E.M., Koo, S.J., Effects of mucilage from yam (Dioscorea batatas Decne) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean J. Food Sci. Technol., 33, 795-801 (2001).
  5. Kang, T.H., Choi, S.Z., Lee, T.H., Son, M.W., Kim, S.Y., Characteristics of antidiabetic effect of Dioscorea rhizoma(1)-Hypoglycemic effect. Korean J. Food & Nutr., 21, 425-429 (2008).
  6. Kwon, C.S., Sohn, H.Y., Kim, S.H., Kim, J.H., Son, K.H., Lee, J.S., Lim, J.K., Kim, J.S., Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem., 67, 1451-1456 (2003). https://doi.org/10.1271/bbb.67.1451
  7. Hou, W.C., Hsu, F.L., Lee, M.H., Yam (Dioscorea batatas) tuber mucilage exhibited antioxidant activities in vitro. Planta Med., 68, 1072-1076 (2002). https://doi.org/10.1055/s-2002-36356
  8. Wanasundera, J.P., Ravindran, G., Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Hum. Nutr., 46, 33-39 (1994). https://doi.org/10.1007/BF01088459
  9. Sautour, M., Mitaine-Offer, A.C., Miyamoto, T., Dongmo, A., Lacaille-Dubois, M.A., Antifungal steroid saponins from Dioscorea cayenensis. Planta Med., 70, 90-92 (2004). https://doi.org/10.1055/s-2004-815467
  10. Kim, M., Gu, M.J., Lee, J.G., Chin, J., Bae, J.S., Hahn, D., Quantitative analysis of bioactive phenanthrenes in Dioscorea batatas decne peel, a discarded biomass from postharvest processing. Antioxidants, 8, 541 (2019). https://doi.org/10.3390/antiox8110541
  11. Kum, E.J., Park, S.J., Lee, B.H., Kim, J.S., Son, K.H., Sohn, H.Y., Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscorea batatas Decne. J. Life Sci., 16, 647-652 (2006). https://doi.org/10.5352/JLS.2006.16.4.647
  12. Lu, Y., Jin, M.H., Park, S.J., Son, K.H., Son, J.K., Chang, H.W., Batatasin, I., A naturally occurring phenanthrene derivative, isolated from tuberous roots of Dioscorea batatas suppresses eicosanoids generation and degranulation in bone marrow derived-mast cells. Biol. Pharm. Bull., 34, 1021-1025 (2011). https://doi.org/10.1248/bpb.34.1021
  13. Choi, E.M., Koo, S.J., Hwang, J.K., Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas). J. Ethnopharmacol., 91, 1-6 (2004). https://doi.org/10.1016/j.jep.2003.11.006
  14. Narula, A., Kumar, S., Bansal, K.C., Srivastava, P.S., In vitro micropropagation, differentiation of aerial bulbils and tubers and diosgenin content in Dioscorea bulbifera. Planta Med., 69, 778-779 (2003). https://doi.org/10.1055/s-2003-42781
  15. Fu, Y.C., Ferng, L.H.A., Huang, P.Y., Quantitative analysis of allantoin and allantoic acid in yam tuber, mucilage, skin and bulbil of the Dioscorea species. Food Chem., 94, 541-549 (2006). https://doi.org/10.1016/j.foodchem.2004.12.006
  16. Drewes, S.E., van Staden, J., Determination of allantoin in Protea seed. Phytochemistry, 14, 751-753 (1975). https://doi.org/10.1016/0031-9422(75)83028-7
  17. Araujo, L.U., Grabe-Guimaraes, A., Mosqueira, V.C.F., Carneiro, C.M., Silva-Barcellos, N.M., Profile of wound healing process induced by allantoin. Acta. Cir. Bras., 25, 460-466 (2010). https://doi.org/10.1590/S0102-86502010000500014
  18. Go, H.K., Rahman, M., Kim, G.B., Na, C.S., Song, C.H., Kim, J.S., Kim, S.J., Kang, H.S., Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes. Nutrients, 7, 8532-8544 (2015). https://doi.org/10.3390/nu7105411
  19. Zhang, Z., Gao, W., Wang, R., Huang, L., Changes in main nutrients and medicinal composition of Chinese yam (Dioscorea opposita) tubers during storage. J. Food Sci. Technol., 51, 2535-2543 (2014). https://doi.org/10.1007/s13197-012-0776-y
  20. Liu, Y., Li, H., Fan, Y., Man, S., Liu, Z., Gao, W., Wang, T., Antioxidant and antitumor activities of the extracts from Chinese yam (Dioscorea opposite Thunb.) flesh and peel and the effective compounds. J. Food Sci., 81, 553-564 (2016).
  21. Chen, M.F., Tsa, J.T., Chen, L.J., Wu, T.P., Yang, J.J., Yin, L.T., Yang, Y.l., Chiang, T.A., Lu, H.L., Wu, M.C., Antihypertensive action of allantoin in animals. Biomed Res. Int., 2014, 690135 (2014). https://doi.org/10.1155/2014/690135
  22. Berthemy, A., Newton, J., Wu, D., Buhrman, D., Quantitative determination of an extremely polar compound allantoin in human urine by LC-MS/MS based on the separation on a polymeric amino column. J. Pharm. Biomed. Anal., 19, 429-434 (1999). https://doi.org/10.1016/S0731-7085(98)00200-3
  23. Czauderna, M., Kowalczyk, J., Quantification of allantoin, uric acid, xanthine and hypoxanthine in ovine urine by high-performance liquid chromatography and photodiode array detection. J. Chromatogr. B: Biomed. Sci. Appl., 744, 129-138 (2000). https://doi.org/10.1016/S0378-4347(00)00239-5
  24. George, S.K., Dipu, M.T., Mehra, U.R., Singh, P., Verma, A.K., Ramgaokar, J.S., Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine. J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci., 832, 134-137 (2006). https://doi.org/10.1016/j.jchromb.2005.10.051
  25. Shingfield, K.J., Offer, N.W., Determination of allantoin in bovine milk by high-performance liquid chromatography. J. Chromatogr. B: Biomed. Sci. Appl., 706, 342-346 (1998). https://doi.org/10.1016/S0378-4347(97)00549-5
  26. Terzuoli, L., Pandolfi, M., Arezzini, L., Pizzichini, M., Marinello, E., Pagani, R., Separation and determination of liver uric acid and allantoin. J. Chromatogr. B: Biomed. Sci. Appl., 663, 143-147 (1995). https://doi.org/10.1016/0378-4347(94)00420-A
  27. Sagara, K., Ojima, M., Suto, K., Yoshida, T., Quantitative determination of allantoin in Dioscorea rhizome and an Oriental pharmaceutical preparation, hachimi-gan, by high-performance liquid chromatography. Planta Med., 55, 93 (1989).
  28. Yoon, K.D., Yang, M.H., Chin, Y.W., Park, J.H., Kim, J.W., Determination of allantoin in Dioscorea Rhizoma by high performance liquid chromatography using cyano columns. Nat. Prod. Sci., 14, 254-259 (2008).
  29. Hwang, I.G., Moon, G.I., Jang, M.I., Kim, S.H., Ko, Y.H., Jo, Y.J., Lee, S.M., Kang, Y.W., Heo, E.J., Im, H.S., Seo, I.W., Kang, T.S., 2016. Guideline on standard procedures for preparing analysis method. Ministry of Food and Drug Safety, Cheongju, Korea, pp. 2-16.
  30. Haghi, G., Arshi, R., Safaei, A., Improved high-performance liquid chromatography (HPLC) method for qualitative and quantitative analysis of allantoin in Zea mays. J. Agric. Food Chem., 56, 1205-1209 (2008). https://doi.org/10.1021/jf0727793
  31. Kwon, J.B., Kim, M.S., Sohn, H.Y., Evaluation of antimicrobial, antioxidant, and antithrombin activities of the rhizome of various Dioscorea species. Korean J. Food Preserv., 17, 391-397 (2010).
  32. Lim, J.S., Hahn, D., Gu, M.J., Oh, J., Lee, J.S., Kim, J.S., Anti-inflammatory and antioxidant effects of 2, 7-dihydroxy-4, 6-dimethoxy phenanthrene isolated from Dioscorea batatas Decne. Appl. Biol. Chem., 62, 29 (2019). https://doi.org/10.1186/s13765-019-0436-2
  33. Lee, W., Jeong, S.Y., Gu, M.J., Lim, J.S., Park, E.K., Baek, M.C., Kim, J.S., Hahn, D., Bae, J.S., Inhibitory effects of compounds isolated from Dioscorea batatas Decne peel on particulate matter-induced pulmonary injury in mice. J. Toxicol. Environ. Health A, 82, 727-740 (2019). https://doi.org/10.1080/15287394.2019.1646174
  34. Jeong, S.Y., Kim, M., Park, E.K., Kim, J.S., Hahn, D., Bae, J.S., Inhibitory functions of novel compounds from Dioscorea batatas Decne peel on HMGB1-mediated septic responses. Biotech. Bioproc. Eng., 25, 1-8 (2020). https://doi.org/10.1007/s12257-019-0382-1