Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Molecular Identification of Cryptosporidium viatorum Infection in a Patient Suffering from Unusual Cryptosporidiosis in West Bengal, India

  • Sardar, Sanjib Kumar (Division of Parasitology, National Institute of Cholera and Enteric Diseases) ;
  • Ghosal, Ajanta (Division of Parasitology, National Institute of Cholera and Enteric Diseases) ;
  • Saito-Nakano, Yumiko (Department of Parasitology, National Institute of Infectious Diseases) ;
  • Dutta, Shanta (Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases) ;
  • Nozaki, Tomoyoshi (Department of Biomedical Chemistry, School of International Health, Graduate School of Medicine, The University of Tokyo) ;
  • Ganguly, Sandipan (Division of Parasitology, National Institute of Cholera and Enteric Diseases)
  • Received : 2021.04.08
  • Accepted : 2021.07.29
  • Published : 2021.08.31
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Abstract

In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.

Keywords

Acknowledgement

The study was supported by a grant from National Institute of Infectious Disease, Tokyo, Japan. The authors would like to thank Dr. Koushik Das for his immense help for providing technical support.

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