Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Validation and measurement uncertainty of HPLC method for simultaneous determination of 10 dyes in adulterated Phellodendron

  • Lim, Suji (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Yun, Choong-In (Lab of Nanobio, Seoul National University of Science and Technology) ;
  • Ko, Kyung Yuk (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Young-Jun (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • Received : 2021.05.31
  • Accepted : 2021.07.01
  • Published : 2021.08.31
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Abstract

As global interest in herbal medicines has increased, the adulteration of herbal medicines has become a critical safety issue. Adulteration with dyes to improve the appearance of low-quality products is of particular concern. This study aimed to develop a high-performance liquid chromatography (HPLC) method to detect dyes added as adulterants to Phellodendron. Samples were analyzed on a C18 column using 50 mM ammonium acetate and acetonitrile as the mobile phase. All calibration curves showed good linearity (r2 ≥0.9999) over the five-point concentration range (1-50 mg/kg). Limit of detection ranged from 0.04-0.35 mg/kg, and limit of quantification ranged from 0.11-1.07 mg/kg. The repeatability and reproducibility for these measurements were 94.2-103.3% and 96.6-103.8% for accuracy and 0.14-2.28 RSD (%) and 0.80-2.37 RSD (%) for precision. Moreover, the measurement uncertainty of the low, medium, and high concentrations for 10 dyes was considered. Thus, this HPLC method is suitable for detecting color adulteration of Phellodendron.

Keywords

Acknowledgement

This research was supported by a grant (20172MFDS231) from Ministry of Food and Drug Safety in 2021.

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