Restorative Dentistry and Endodontics

  • 제46권1호
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  • Pages.1.1-1.13
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  • 2021
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue

  • Ranjdar Mahmood Talabani (Department of Conservative Dentistry, University of Sulaimani) ;
  • Balkees Taha Garib (Department of Oral Diagnosis, University of Sulaimani) ;
  • Reza Masaeli (Department of Dental Biomaterial, Tehran University of Medical Sciences) ;
  • Kavosh Zandsalimi (Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran) ;
  • Farinaz Ketabat (Division of Biomedical Engineering, University of Saskatchewan)
  • 투고 : 2020.01.24
  • 심사 : 2020.08.07
  • 발행 : 2021.02.28
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초록

Objectives: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats. Materials and Methods: Forty-five silicon tubes containing the tested materials and 15 empty tubes (serving as a control group) were subcutaneously implanted into the backs of 15 Wistar rats. At 1, 4, and 8 weeks after implantation, the animals were euthanized (n = 5 animals/group), and the silicon tubes were removed with the surrounding tissues. Histopathological tissue sections were stained with von Kossa stain to assess mineralization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were also used to assess the chemical components of the surface precipitates deposited on the implant and the pattern of calcium and phosphorus distribution at the material-tissue interface. The calcium-to-phosphorus ratios were compared using the non-parametric Kruskal-Wallis test at a significance level of 5%. Results: The von Kossa staining showed that both BD and ESRRM putty induced mineralization starting at week 1; this mineralization increased further until the end of the study. In contrast, MM-MTA induced dystrophic calcification later, from 4 weeks onward. SEM/EDX showed no statistically significant differences in the calcium- and phosphorus-rich areas among the 3 materials at any time point (p > 0.05). Conclusions: After subcutaneous implantation, biomineralization of the 3-calcium silicate-based cements started early and increased over time, and all 3 tested cements generated calcium- and phosphorus-containing surface precipitates.

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과제정보

The authors are indebted to Mrs. Ghodsie Morsali for her kind help and technical assistance.

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