Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Colitic Effects of Herbal Medicine HPL-01 on Dextran Sulfate Sodium Induced Ulcerative Colitis in Rat

해동피를 포함한 한약제제 HPL-01의 Dextran sodium sulfate 유도 궤양성 대장염 완화 효과

  • Received : 2020.12.29
  • Accepted : 2021.04.13
  • Published : 2021.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to investigate the anti-inflammatory effects of herbal complex HPL-01 on dextran sodium sulfate(DSS) induced ulcerative colitis in rat. Adult male Sprague-Dawley rats were randomized and divided into six groups. Five groups, excluding the normal group, were orally administered orally HPL-01(50, 100, or 200 mg/kg) for 21 days, and acute colitis was induced during the last 7 days by 4% DSS in the drinking water. The HPL-01 administered DSS-treated rats exhibited significantly reduced colon macroscopic damage index and increased body weight and colonic length 7 days after DSS treatment. Additionally, these rats showed lower serum levels of the pro-inflammatory cytokines TNF-α and IL-6 than those treated only with DSS. HPL-01(100 or 200 mg/kg) also attenuated the DSS-induced increase in the number of white blood cells, granulocytes, and mid cells and improved intestinal damage. Taken together, these results suggest that HPL-01 is a promising anti-inflammatory agent that may be in the treatment of colitis.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 한국산업기술진흥원의 "국가융합단지 연계 상용화(R&D, P0009978)"사업의 지원을 받아 수행된 연구임.

References

  1. Sands, Bruce E. From symptom to diagnosis: clinical distinctions among various forms of intestinal inflammation. Gastro. 2004;126:1518-32. https://doi.org/10.1053/j.gastro.2004.02.072
  2. on ulcerative colitis in DSS-induced ICR Mice. J Physiol & Pathol Korean Med 2016;30:439-46. https://doi.org/10.15188/kjopp.2016.12.30.6.439
  3. Blumberg RS, Saubermann LJ, Strober W. Animal models of mucosal inflammation and their relation to human inflammatory bowel disease. Curr Opin Immunol. 1999;11:648-56. https://doi.org/10.1016/S0952-7915(99)00032-1
  4. Jiminez JA, Uwiera TC, Inglis GD, Uwiera RR. Animal models to study acute and chronic intestinal inflammation in mammals. Gut Pathog. 2015;7:29. https://doi.org/10.1186/s13099-015-0076-y
  5. Klotz U. Colonic targeting of aminosalicylates for the treatment of ulcerative colitis. Dig Liver Dis. 2005;37:381-8. https://doi.org/10.1016/j.dld.2004.12.011
  6. Hanauer SB, Sparrow M. Therapy of ulcerative colitis. Curr Opin Gastro. 2004;20:345-50. https://doi.org/10.1097/00001574-200407000-00008
  7. Hyun TK, Kim JS. The pharmacology and clinical properties of Kalopanax pictus. J Med Plants Res. 2009;3:613-20.
  8. Choi J, Huh K, Kim SH, Lee KT, Park HJ, Han YN. Antinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals. J Ethnopharmacol. 2002;79:199-204. https://doi.org/10.1016/S0378-8741(01)00383-X
  9. Hamauzu Y, Kishida H, Yamazaki N. Gastroprotective property of Pseudocydonia sinensis fruit jelly on the ethanol-induced gastric lesions in rats. J Functional Foods. 2018;48:275-82. https://doi.org/10.1016/j.jff.2018.07.026
  10. Sham TT, Yuen ACY, Ng YF, Chan CO, Mok DKW, Chan SW. A review of the phytochemistry and pharmacological activities of raphani semen. Evid.-Based Complementary Altern. Med. 2013;636194. https://doi.org/10.1155/2013/636194
  11. Kim CM, Lee YC, Lee JC. The effects of sinapis semen, raphani semen, and mixture decoction on the asthmatic mouse model. Korea J Herb 2013;28:15-23.
  12. Hur IH, Sim SY, Kim KJ. Anti-inflammatory effect of various solvent extract from Atractylodes japonica on lipopolysaccharide-induced inflammation in BV2 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2007;20:36-46.
  13. Lim DJ, Choi YH, Hwang WD. Induction of apoptotic cell death by ethanolic extract of aconiti tuber in human hepatocarcinoma cells. Cancer Prevent Res. 13.2 2008;13:142-52.
  14. McCafferty DM, Sharkey KA, Wallace JL. Beneficial effects of local or systemic lidocaine in experimental colitis. Am J Physiol Gastrointest Liver Physiol. 1994;266:G560-7. https://doi.org/10.1152/ajpgi.1994.266.4.G560
  15. Akiyama S, Nesumi A, Maeda-Yamamoto M, Uehara M, Murakami A. Effects of anthocyanin-rich tea "Sunrouge" on dextran sodium sulfate-induced colitis in mice. Biofactors. 2012;38:226-33. https://doi.org/10.1002/biof.1008
  16. Marocchi M, Sartini A, Roda E. Cytokine Networks in Ulcerative Colitis. Ulcers. 2011;391787-5.
  17. Nakajima H, Munakata A, Yoshida Y. Adverse effects of sulfasalazine and treatment of ulcerative colitis with mesalazine. J Gastroenterol. 1995;30:115-7.
  18. Schroeder KW. Is mesalamine safe?. Gastroenterol Hepatol (N Y). 2007;3:878-9.
  19. Sands BE. Inflammatory bowel disease: past, present, and future. J Gastroenterol. 2007;42:16-25. https://doi.org/10.1007/s00535-006-1995-7
  20. Baumgart DC, Carding SR. Inflammatory bowel disease: cause and immunobiology. Lancet. 2007;369:1627-40. https://doi.org/10.1016/S0140-6736(07)60750-8
  21. Kim HS, Berstad A. Experimental colitis in animal models. Scand J gastroenterol. 1992;27:529-37. https://doi.org/10.3109/00365529209000116
  22. Ardizzone S, Porro GB. Biologic therapy for inflammatory bowel disease. Drugs. 2005;65:2253-86. https://doi.org/10.2165/00003495-200565160-00002
  23. Adegbola SO, Sahnan K, Warusavitarne J, Hart A, Tozer P. Anti-TNF Therapy in Crohn's Disease. IJMS. 2018;19:2244. https://doi.org/10.3390/ijms19082244
  24. Fiocchi C. Inflammatory bowel disease: etiology and pathogenesis. Gastroenterol. 1998;115:182-205. https://doi.org/10.1016/S0016-5085(98)70381-6
  25. Melgar S, Engstrom K, Jagervall A, Martinez V. Psychological stress reactivates dextran sulfate sodium-induced chronic colitis in mice. Stress. 2008;11:348-62. https://doi.org/10.1080/10253890701820166