Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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The Anti-inflammatory Effects of Golden Apple Snail (Pomacea canaliculata) in Reflux Esophagitis Model

왕우렁이 추출물의 역류성 식도염 억제 효과

  • 남현화 (한국한의학연구원 한약자원연구센터) ;
  • 류승목 (한국한의학연구원 한약자원연구센터) ;
  • 양선규 (한국한의학연구원 한약자원연구센터) ;
  • 김욱진 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터)
  • Received : 2020.10.06
  • Accepted : 2020.12.23
  • Published : 2021.02.27
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Abstract

Reflux esophagitis (RE) is a common gastrointestinal disease observed at all ages, which seriously affects the quality of life. In this study, we investigated the anti-inflammatory effects of Pomacea canaliculata extract (PCE) using the experimental RE rat model. RE was induced by a surgical procedure. The rats were randomly divided into 4 groups: normal group, RE group, PCE group (RE treated with PCE, 100 mg/kg), positive control group (RE treated with ranitidine, 40 mg/kg). We performed the histological examination and measured the expression of tight junction complex and inflammatory mediators using western blot analysis. The phenotypes of RE were attenuated by PCE treatment. PCE administration significantly reduced esophageal mucosal damage and protected tight junction confirmed by claudin-5. Furthermore, PCE treatment reduced inflammatory reaction by inhibiting the expression of COX-2 and TNF-α. PCE treatment, also, reduced translocation of NF-κB into nuclear and IκB-α phosphorylation at the same time. Our findings indicate that PCE has the potential as a novel therapeutic agent to inhibit RE by protecting mucosal damage and regulating inflammatory reactions mediated by NF-κB signaling.

본 결과를 통해, 왕우렁이 추출물의 역류성 식도염 모델에서 위 내용물의 역류로 인한 조직손상에 대한 보호효과를 확인하였다. 식도 점막의 육안 소견과 조직병리학적 분석 결과 점막의 괴사, 염증세포의 침윤 등의 증상이 완화됨을 확인하였으며, 상피조직의 tight junction을 보호하여 위산과 펩신으로부터 점막을 보호하는 것을 확인하였다. 이러한 왕우렁이 추출물의 식도 보호효과는 NF-κB 기전의 조절을 통해 점막의 염증반응을 억제하여 나타나는 것으로 생각된다. 이상의 결과를 종합하였을 때, 왕우렁이 추출물이 역류성 식도염 완화를 위한 기능성 소재로서의 가능성을 가지는 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 한국한의학연구원 '지속가능한 한약표준자원 활용 기술' (KSN2012320) 사업 지원으로 수행되었으며, 이에 감사드립니다.

References

  1. Altomare, A., M. P. L. Guarino, S. Cocca, S. Emerenziani, and M. Cicala. 2013. "Gastroesophageal reflux disease: update on inflammation and symptom perception", World J. Gastroenterol. 19(39): 6523-6528. https://doi.org/10.3748/wjg.v19.i39.6523
  2. Bae, K. G. 2006. Current Situation of Agricultural Organic Products and Organic Processing Food. Food Sci. & Industry. 39(3): 35-51.
  3. Biancani, P., U. D. Sohn, H. G. Rich, K. M. Harnett, and J. Behar. 1997. Signal transduction pathways in oesophageal and lower oesophageal sphincter circular muscle. Am. J. Med. 103, pp. 23S-28S. https://doi.org/10.1016/S0002-9343(97)00316-1
  4. Buttar, N. S., K. K. Wang, M. A. Anderson, R. A. Dierkhising, R. J. Pacifico, K. K Krishnadath, and L. S. Lutzke. 2002. The effect of selective cyclooxygenase-2 inhibition in Barrett's esophagus epithelium: an in vitro study. J. Natl. Cancer Inst. 94(6): 422-429. https://doi.org/10.1093/jnci/94.6.422
  5. Castoldi, A., C. Favero de Aguiar, P. M. Moraes-Vieira, and N. Olsen Saraiva Camara. 2015. They must hold tight: junction proteins, microbiota and immunity in intestinal mucosa. Curr. Protein Pept. Sci. 16(7): 655-671. https://doi.org/10.2174/1389203716666150630133141
  6. DeMeester, T. R., J. A. Wernly, and G. H. Bryant. 1979. Clinical and in vitro analysis of determinants of gastroesophageal competence. A study of the principles of antireflux surgery. Am. J. Surg. 137(1): 39-46. https://doi.org/10.1016/0002-9610(79)90008-4
  7. Jabri, M. A., H. Tounsi, A. Abdellaoui, L. Marzouki, and H. Sebai. 2018. Protective effects of Artemisia campestris extract against gastric acid reflux-induced esophageal mucosa injuries. Pathophysiology. 25(1): 63-69. https://doi.org/10.1016/j.pathophys.2018.01.001
  8. Jang, M. W. and S. W. Lim. 2013. Experimental Study for Effect of Banhasasim-tang on Mice with Reflux Esophagitis. Korean J. Orient. Int. Med. 34(4): 362-374.
  9. Jung, H. K., S. J. Hong. Y. J. Jo. S. W. Jeon. Y. K. Cho. K. J. Lee. J. S. Lee. H. J. Park. E. S. Shin. S. H. Lee. S. U. Han. and Korean Society of Neurogastroenterology and Motility. 2012. Updated guidelines 2012 for gastroesophageal reflux disease. Korean J. Gastroenterol. 60(4): 195-218. https://doi.org/10.4166/kjg.2012.60.4.195
  10. Lee, D. H. and H. H. Lee. 2016. The effect of food service consumers' organic selection attributes on satisfaction. Int. J. Cult. Tour. Hosp. Res. 30(5): 97-109.
  11. Lee, S. B., M. H. Koh, Y. E. Na, and J. H. Kim. 2002. Physiological and Ecological Characteristics of the Apple Snails. Korean J. Environ. Agri. 21(1): 50-56. https://doi.org/10.5338/KJEA.2002.21.1.050
  12. Liu, C., Y. Zhang, Y. Ren, H. Wang, S. Li, F. Jiang, L. Yin, X. Qiao, G. Zhang, W. Qian, B. Liu, and W. Fan. 2018. The genome of the golden apple snail Pomacea canaliculata provides insight into stress tolerance and invasive adaptation. Gigascience. 7(9): giy101. https://doi.org/10.1093/gigascience/giy101
  13. Okimoto, K., M. Arai, H. Ishigami, K. Saito, S. Minemura, D. Maruoka, T. Matsumura, T. Nakagawa, T. Katsuno, M. Suzuki, Y. Nakatani, and O. Yokosuka. 2018. A Prospective Study of Eosinophilic Esophagitis and the Expression of Tight Junction Proteins in Patients with Gastroesophageal Reflux Disease Symptoms. Gut Liver. 12(1): 30-37. https://doi.org/10.5009/gnl16600
  14. Osadchuk, A. M., I. L. Davydkin, T. A. Gricenko, and M. A. Osadchuk. 2019. Gastroesophageal reflux disease and esophagitis associated with the use of drugs: the modern state of the problem. Ter. Arkh. 91(8): 135-140.
  15. Pawlik, M., R. Pajdo, S. Kwiecien, A. Ptak-Belowska, Z. Sliwowski, M. MazurkiewiczJanik, S. J. Konturek, W. W. Pawlik, and T. Brzozowski. 2011. Nitric Oxide (NO)-releasing Aspirin Exhibits a Potent Esophagoprotection in Experimental Model of Acute Reflux Esophagitis. Role of Nitric Oxide and Proinflammatory Cytokines. J. Physiol. Pharmacol. 62(1): 75-86.
  16. Piazuelo, E., P. Jimenez, and A. Lanas. 2003. COX-2 Inhibition in Esophagitis, Barrett's Esophagus and Esophageal Cancer. Curr. Pharm. Des. 9(27): 2267-2280. https://doi.org/10.2174/1381612033454009
  17. Rao, C. V. and M. Vijayakumar. 2008. Effect of Quercetin, Flavonoids and Alpha-Tocopherol, an Antioxidant Vitamin, on Experimental Reflux Oesophagitis in Rats. Eur. J. Pharmacol. 589(1-3): 233-238. https://doi.org/10.1016/j.ejphar.2008.04.062
  18. Singh, P., N. Singh, S. Sengupta, and G. Palit. 2012. Ameliorative effects of Panax quinquefolium on experimentally induced reflux oesophagitis in rats, Indian J. Med. Res. 135(3): 407-413.
  19. Souza, R. F., K. Shewmake, D. G. Beer, B. Cryer, and S. J. Spechler SJ. 2000. Selective inhibition of cyclooxygenase-2 suppresses growth and induces apoptosis in human esophageal adenocarcinoma cells. Cancer Res. 60(20): 5767-5772.
  20. Watson, T. J. and J. H. Peters. 2005. Lower esophageal sphincter injections for the treatment of gastroesophageal reflux disease. Thorac. Surg. Clin. 15(3): 405-415. https://doi.org/10.1016/j.thorsurg.2005.04.001
  21. Wu, L., T. Oshima, M. Li, T. Tomita, H. Fukui, J. Watari, and H. Miwa. 2018. Filaggrin and tight junction proteins are crucial for IL-13-mediated esophageal barrier dysfunction. Am. J. Physiol. Gastrointest. Liver Physiol. 315(3): G341-G350.