한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제49권1호
  • /
  • Pages.88-94
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  • 2021
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Galactose에 순치한 Saccharomyces cerevisiae를 이용하여 꼬시래기(Gracilaria verrucosa)로부터 바이오에탄올 생산

Bioethanol Production from Gracilaria verrucosa Using Saccharomyces cerevisiae with Adaptive Evolution

  • 양지원 (부경대학교 생물공학과) ;
  • 박유림 (부경대학교 생물공학과) ;
  • 정귀택 (부경대학교 생물공학과) ;
  • 김성구 (부경대학교 생물공학과)
  • Yang, Ji Won (Department of Biotechnology, Pukyong National University) ;
  • Park, Yu Rim (Department of Biotechnology, Pukyong National University) ;
  • Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
  • 투고 : 2020.09.09
  • 심사 : 2020.11.24
  • 발행 : 2021.03.28
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초록

해조류 중 홍조류인 꼬시래기(G. verrucosa)로부터 효모를 이용한 발효를 위해 열산가수분해, 효소당화 및 에탄올 발효수율 향상을 검토하고, 기존의 혼합당의 흡수효율을 높이기 위해 고농도 당 순치를 수행하였다. 열산가수분해는 200 mM 황산(H2SO4)을 이용하여 10% (w/v)의 꼬시래기(G. verrucosa)의 슬러리, 130℃의 온도에서 60분 동안 열산가수분해를 수행하였다. 또한 wild type 효모와 고농도 galactose에 순치(adaptive evolution)된 효모를 이용한 발효를 실시한 결과, wild type 효모의 경우 발효 144시간에 8.5 g/l 에탄올 발효로 에탄올수율계수 YEtOH = 0.19와 galactose에 순치된 효모의 경우 21.5 g/l 에탄올 발효로 에탄올수율계수 YEtOH = 0.50을 나타내었다. 이러한 연구결과는 해양 바이오매스인 해조류로부터 바이오 연료의 효율적인 생산방법을 제공할 수 있을 것으로 판단된다.

The seaweed, Gracilaria verrucosa (red seaweed) was fermented to produce bioethanol. Optimal thermal acid hydrolysis conditions were determined as 200 mM H2SO4 and 10% (w/v) seaweed slurry at 130℃ for 60 min yielding 47.5% of pretreatment efficiency (Ep). After the thermal acid hydrolysis, enzymatic saccharification was carried out with 16 U/ml Viscozyme L, Cellic CTec2 or mixture of Viscozyme L and Cellic CTec2 to G. verrucosa hydrolysates. Enzymatic saccharifications with Viscozyme, Cellic CTec2 or mixture of those yielded 7.3 g/l glucose with efficiency of saccharification, Es = 34.9%, 11.6 g/l glucose with Es = 64.4% and the mixture of those 9.6 g/l glucose with Es = 56.6%, respectively. Therefore, based on the Es value, Cellic CTec2 was selected for the optimal enzyme for enzymatic saccharification of G. verrucosa hydrolysate. The ethanol productions with non-adapted S. cerevisiae CEN-PK2 (wild type) and S. cerevisiae CEN-PK2 with adaptive evolution to galactose produced 8.5 g/l ethanol with YEtOH = 0.19 and 21.5 g/l ethanol with YEtOH = 0.50 at 144 h, respectively. From these results, the ethanol production by S. cerevisiae with adaptive evolution showed high concentration of ethanol production using G. verrucosa as a substrate.

키워드

과제정보

This work was supported by a Research Grant of Pukyong National University (year 2019).

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