한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제36권1호
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  • Pages.9-16
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  • 2021
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Immobilization stress increased cytochrome P450 1A2 (CYP1A2) expression in the ovary of rat

  • Hwang, Jong-Chan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Hwan-Deuk (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Byung-Joon (College of Veterinary Medicine, Kyungpook National University) ;
  • Jeon, Ryoung-Hoon (College of Veterinary Medicine, Gyeongsang National University) ;
  • Baek, Su-Min (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Seoung-Woo (College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, Min (College of Veterinary Medicine, Kyungpook National University) ;
  • Bae, Seul-Gi (College of Veterinary Medicine, Kyungpook National University) ;
  • Yun, Sung-Ho (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Jin-Kyu (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Young-Sam (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Seung-Joon (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Won-Jae (College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 2021.02.10
  • 심사 : 2021.03.08
  • 발행 : 2021.03.31
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초록

Under the stressed condition, a complex feedback mechanism for stress is activated to maintain homeostasis of the body and secretes several stress hormones. But these stress hormones impair synthesis and secretion of the reproductive hormones, followed by suppression of ovarian function. Cytochrome P450 1A2 (CYP1A2) plays a major role in metabolizing exogenous substances and endogenous hormones, and its expression is recently identified at not only the liver but also several organs with respect to the pancreas, lung and ovary. Although the expression of CYP1A2 can be also affected by several factors, understanding for the changed pattern of the ovarian CYP1A2 expression upon stress induction is still limited. Therefore, CYP1A2 expression in the ovaries from immobilization stress-induced rats were assessed in the present study. The stress-induced rats in the present study exhibited the physiological changes in terms of increased stress hormone level and decreased body weight gains. Under immunohistological observation, the ovarian CYP1A2 expression in both control and the stressed ovary was localized in the antral to pre-ovulatory follicles. However, its expression level was significantly (p < 0.01) higher in the stress-induced group than control group. In addition, stress-induced group presented more abundant CYP1A2-positive follicles (%) than control group. Since expression of the ovarian CYP1A2 was highly related with follicle atresia, increased expression of CYP1A2 in the stressed ovary might be associated with changes of the ovarian follicular dynamics due to stress induction. We hope that these findings have important implications in the fields of the reproductive biology.

키워드

과제정보

This work was supported by a grant from the National Research Foundation (NRF) of Korea, funded by the government of the Republic of Korea (NRF-2020R1F1A1076723).

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