Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperature

  • Kim, Seung Min (Mokpo Regional Office, National Fishery Products Quality Management) ;
  • Lee, Da Won (Aquatic Life Disease Control Division, National Fishery Products Quality Management) ;
  • Kim, You Jeong (Central Research Institute, Woogen B&G) ;
  • Jun, Lyu Jin (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Park, Hyun Kyung (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Kim, Ye Ji (Department of Aquatic Biomedical Science, Jeju National University) ;
  • Jeong, You Yong (Central Research Institute, Woogen B&G) ;
  • Lee, Sung Ho (Central Research Institute, Woogen B&G) ;
  • Kwon, Mun Gyeong (Aquatic Life Disease Control Division, National Fishery Products Quality Management) ;
  • Jeong, Joon Bum (Department of Aquatic Biomedical Science, Jeju National University)
  • Received : 2021.03.09
  • Accepted : 2021.03.26
  • Published : 2021.04.30
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Abstract

In this study, a fish metabolic accelerator (a combination of butaphosphan and cyanocobalamin [BPC]) was injected into the muscle of the olive flounder, Paralichthys olivaceus, to investigate its effect on immunity and stress in fish maintained at low temperatures. A single dose of BPC was injected (100 mg/kg body weight) into the olive flounder, and its immunity and stress were observed after one and two weeks. Immunity tests revealed the presence of lysozyme (LZM), nitroblue tetrazolium (NBT), myeloperoxidase (MPO), anti-protease (AP), glutathione peroxidase (GPx), and total immunoglobulin (TIg). BPC injection was found to increase immunity activity compared to the control group. In particular, there was significantly high GPx activity. There was similarly high activity for MPO and GPx in the first week following the injection, followed by significant differences between the BPC-injected and control groups in the second week. There was a reduced low water-temperature stress response in the BPC-injected fish, as evidenced by the cortisol and glucose levels of the control and BPC groups. Lower levels were also observed in the BPC group than the control group during the second week. Cortisol levels were significantly lower in the BPC group than the control group. Histological examinations were conducted in the first and second weeks after the intramuscular injection of the recommended BPC dose to confirm the safety of BPC in aquaculture. There were no abnormalities observed in any tissue samples. This study confirms that the injection of BPC is safe even when used in a culture situation. BPC helps relieve stress and improves non-specific immune responses (innate immunity) in the olive flounder.

Keywords

Acknowledgement

This work was supported by a grant from the National Fishery Products Quality Management Service and the research grant of Jeju National University in 2020.

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