Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Gynostemma pentaphyllum Callus Extract on Anti-inflammation, Anti-allergy, and Alleviation of Keratinocyte Damage Caused by S. aureus

돌외 캘러스 추출물의 항염, 항알러지 및 S. aureus에 의한 각질세포 손상 완화 효능

  • Received : 2020.12.03
  • Accepted : 2021.05.21
  • Published : 2021.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to identify the efficacy related to skin barrier functions such as anti-inflammatory, anti-allergic, cornified envelope formation and the alleviation effect of keratinocyte damage caused by S. aureus using the callus extract, which was induced and extracted from the leaves of Gynostemma pentaphyllum in Ulleungdo Island. In order to confirm the anti-inflammatory effect of the G. pentaphyllum callus extract on the skin, the expression of inflammatory cytokines was investigated in primary epidermal keratinocytes (HEKa) activated with PAR-2 agonist, and the G. pentaphyllum callus extract showed IL-8, IL-25, and TSLP production inhibitory effect. β-Hexosaminidase assay using RBL-2H3 cells was performed to confirm the anti-allergic efficacy, and G. pentaphyllum callus extract showed the effect of inhibiting the release of β-hexosaminidase. In addition, G. pentaphyllum callus extract showed the cornified envelope formation effect in HaCaT cells, and through the co-culture experiment with HaCaT cells and S. aureus, it showed alleviation effect of keratinocyte damage caused by S. aureus. Therefore, G. pentaphyllum callus extract is considered to be a useful cosmetic material for improving skin barrier with anti-inflammatory, anti-allergic and alleviation effect of keratinocyte damage caused by S. aureus.

본 연구는 울릉도 돌외(Gynostemma pentaphyllum) 잎으로부터 캘러스 유도 및 추출한 후, 피부장벽 기능과 관련된 항염증, 항알러지, 각질세포외막 형성, S. aureus에 의한 각질형성세포 손상 완화 효능을 확인하고자 수행되었다. 돌외 캘러스 추출물의 피부에 대한 항염증 효능을 확인하기 위해, PAR-2 agonist로 활성화된 primary epidermal keratinocyte (HEKa)에서 염증성 사이토카인들의 발현을 확인한 결과, 돌외 캘러스 추출물은 IL-8, IL-25, TSLP 발현을 저해하는 효능이 있는 것을 확인하였다. RBL-2H3 세포를 이용한 β-hexosaminidase assay 시험을 통해 항알러지 효능을 확인한 결과, β-hexosaminidase 방출을 억제하는 효과를 보였다. 또한, 돌외 캘러스 추출물은 HaCaT 세포에서 각질세포외막(cornified envelope) 형성 효과가 있음을 확인하였고, HaCaT 세포와 S. aureus 공배양 실험을 통해 S. aureus에 의한 각질형성세포 생존율 감소를 완화하는 효능이 있음을 확인하였다. 본 연구 결과를 종합해보면, 울릉도 돌외 캘러스 추출물은 항염, 항알러지, S. aureus에 의한 각질세포 손상 완화 효능에 유효한 소재의 가능성을 확인하였고, 피부장벽 개선을 위한 화장품 소재로서 활용 가치가 있을 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 보건의료기술 연구개발사업 지원에 의하여 이루어진 것입니다(과제고유번호: HP20C0029).

References

  1. B. Wang, M. Li, H. Gao, X. Sun, B. Gao, Y. Zhang, and L. Yu, Chemical composition of tetraploid Gynostemma pentaphyllum gypenosides and their suppression on inflammatory response by NF-κB/MAPKs/AP-1 signaling pathways, Food Sci. Nutr., 8(2), 1197 (2020). https://doi.org/10.1002/fsn3.1407
  2. F. Aktan, S. Henness, B. D. Roufogalis, and A. J. Ammit, Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-κB-mediated iNOS protein expression, Nitric Oxide, 8(4), 235 (2003). https://doi.org/10.1016/S1089-8603(03)00032-6
  3. D. Samec, L. V.-Zulj, S. Martinez, J. Gruz, A. Piljac, and J. P.-Zegarac, Phenolic acids significantly contribute to antioxidant potency of Gynostemma pentaphyllum aqueous and methanol extracts, Ind. Crops Prod., 84, 104 (2016). https://doi.org/10.1016/j.indcrop.2016.01.035
  4. E. H. Choi and N. Y. Yoon, Pathogenesis of atopic dermatitis, J. Korean Medical Assoc., 57(3), 218 (2014). https://doi.org/10.5124/jkma.2014.57.3.218
  5. D. Redhu, K. Franke, V. Kumari, W. Francuzik, M. Babina, and M. Worm, Thymic stromal lymphopoietin production induced by skin irritation results from concomitant activation of protease-activated receptor 2 and interleukin 1 pathways, Br. J. Dermatol., 182(1), 119 (2020).
  6. S. R. Wilson, L. T. Lydia, L. M. Batia, K. Beattie, G. E. Katibah, S. P. McClain, M. Pellegrino, D. M. Estandian, and D. M. Bautista, The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch, Cell, 155(2), 285 (2013). https://doi.org/10.1016/j.cell.2013.08.057
  7. J. M. Leyva-Castillo, P. Hener, H. Jiang, and M. Li, TSLP produced by keratinocytes promotes allergen sensitization through skin and thereby triggers atopic march in mice, J. Invest. Dermatol., 133(1), 154 (2013). https://doi.org/10.1038/jid.2012.239
  8. J. H. Ju, C. H. Lim, J. H. Kim, J. P. Lee, K. H. Son, S. K. Hong, T. R. Kwon, M. Chang, D. S. Kim, H. S. Yoon, and K. L. Park, Establishment of screening and test methods of atopic dermatitis using in vitro systems, J. Altern. Anim. Exp., 4(1), 31 (2010).
  9. R. H. Guo, J. U. Park, S. J. Jo, J. H. Ahn, J. H. Park, J. Y. Yang, S. S. Lee, M. J. Park, and Y. R. Kim, Anti-allergic inflammatory effects of the essential oil from fruits of Zanthoxylum coreanum Nakai, Front. Pharmacol., 12(9), 1441 (2018).
  10. H. H. Kong, J. Oh, C. Deming, S. Conlan, E. A. Grice, M. A. Beatson, E. Nomicos, E. C. Polley, H. D. Komarow, P. R. Murray, M. L. Turner, and J. A. Segre, Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis, Genome Res., 22(5), 850 (2012). https://doi.org/10.1101/gr.131029.111
  11. T. Nakatsuji, T. H. Chen, A. M. Two, K. A. Chun, S. Narala, R. S. Geha, T. R. Hata, and R. L. Gallo, Staphylococcus aureus exploits epidermal barrier defects in atopic dermatitis to trigger cytokine expression, J. Invest. Dermatol., 136(11), 2192 (2016). https://doi.org/10.1016/j.jid.2016.05.127
  12. J. B. Travers, D. A. Norris, and D. Y. Leung, The keratinocyte as a target for Staphylococcal bacterial toxins, J. Investig. Dermatol. Symp. Proc., 6(3), 225 (2001). https://doi.org/10.1046/j.0022-202x.2001.00045.x
  13. S. B. Park, Ph. D. Dissertation, Dong-eui Univ., Busan, Korea (2011).
  14. Y. J. Jung, Y. S. Jeon, H. J. Kim, K. S. Kang, Y. K. Kim, and S. N. Kim, Anti-allergic and anti-inflammatory effect of Leonurus sibiricus seed ethyl acetate fractions, Yakhak Hoeji, 58(5), 294 (2014).
  15. T. Hasegawa, H. Shimada, T. Uchiyama, O. Ueda, M. Nakashima, and Y. Matsuoka, Dietary glucosylceramide enhances cornified envelope formation via transglutaminase expression and involucrin production, Lipids, 46(6), 529 (2011). https://doi.org/10.1007/s11745-011-3546-0
  16. L. L. Zhou, Z. X. Lin, K. P. Fung, C. T. Che, M. Zhao, C. H. K. Cheng, and Z. Zuo, Ethyl acetate fraction of Radix rubiae inhibits cell growth and promotes terminal differentiation in cultured human keratinocytes, J. Ethnopharmacol., 142(1), 241 (2012). https://doi.org/10.1016/j.jep.2012.04.051