Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Pathologic studies for porcine pleuropneumonia and serotypes of the agents in Jeju

제주지역 돼지 흉막폐렴에 대한 병리학적 연구 및 원인체의 혈청형

  • Kim, Ki-Seung (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Yang, Hyoung-Seok (Jeju Self-Governing Provincial Veterinary Research Institute) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 김기승 (제주대학교 수의과대학 및 수의과학연구소) ;
  • 양형석 (제주특별자치도 동물위생시험소) ;
  • 김재훈 (제주대학교 수의과대학 및 수의과학연구소)
  • Received : 2021.06.18
  • Accepted : 2021.06.21
  • Published : 2021.06.30
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Abstract

Actinobacillus (A.) pleuropneumoniae is the etiological agent of a porcine pleuropneumonia and have great economic importance to the global swine industry. For recent 5 years, a total of 50 pleuropneumonia cases of 24 pig farms were selected from pig lungs submitted to the College of Veterinary Medicine, Jeju National University using polymerase chain reaction (PCR) analysis. Collected lungs were fixed in 10% neutral phosphate-buffered formalin and processed for histological examination. Serotypes of A. pleuropneumoniae in pneumonic lesions were analyzed by PCR methods. And the antimicrobial susceptibility of A. pleuropneumoniae isolates was determined by a disc diffusion test. Grossly, unilateral distribution of hemorrhagic or necrotic pneumonic lesions was more common than bilateral distribution in lungs. In peracute or acute cases, histopathologic changes were characterized by necrosis, hemorrhage, neutrophils infiltration, vascular thrombosis, widespread edema and fibrinous exudates. Following the acute response, macrophage infiltration, marked fibrosis around zonal necrotic areas, and marked fibrous pleuritis were characteristic in chronic cases. A total of 50 pleuropneumonia were associated with A. pleuropneumoniae serotype 5 in 46 cases (92%), serotype 2 in 3 cases (6%), and both 2 and 5 in 1 case (2%). More than 90% of collected isolates showed high sensitivity to ceftiofur, amoxicillin, and colistin. However, ampicillin, penicillin, and tylosin showed low susceptibility. The results of this study demonstrated that A. pleuropneumoniae serotype 5 was predominant at porcine pleuropneumonia cases in Jeju.

Keywords

Acknowledgement

This work was supported by the 2021 education, research and student guidance grant funded by Jeju National University.

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