Medical Lasers

Korean Society for Laser Medicine and Surgery (대한의학레이저학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Solcoseryl in Corneal Alkali Burn Rat Model

  • Kim, Hoon (Department of Ophthalmology, College of Medicine, Dankook University) ;
  • Kim, Hong-Bee (Department of Ophthalmology, College of Medicine, Dankook University) ;
  • Seo, Jae-Hwi (Department of Ophthalmology, College of Medicine, Dankook University) ;
  • Lee, Dong Cho (Department of Ophthalmology, College of Medicine, Dankook University) ;
  • Cho, Kyong Jin (Department of Ophthalmology, College of Medicine, Dankook University)
  • Received : 2020.11.27
  • Accepted : 2021.02.19
  • Published : 2021.03.31
⟨ Previous Next ⟩

Abstract

Background and Objectives Ocular alkali burns cause severe damage to the ocular tissues and vision loss. Solcoseryl is a standardized calf blood extract that normalizes the metabolic disturbance and aids in maintaining the chemical and hormonal balance and has been used to treat burns in various tissues. This study examined the effects of Solcoseryl on a rat corneal alkali burn model. Materials and Methods Twenty rats were assigned randomly to four equal groups, including alkali burn, hyaluronic acid, Solcoseryl eyedrop, and Solcoseryl gel. A corneal alkali burn was induced by a NaOH-soaked paper disc. The treatments were given twice a day, every day. The wound area was measured after 24 and 48 hours, and the degree of neovascularization and corneal opacity were scored every week. The rats were sacrificed after three weeks for immunohistochemistry (IHC) to compare the level of inflammatory cytokines, IL-1β, IL-6, and TNF-α. The thickness of the retinal layers was compared to observe any changes in the retina. Results The use of Solcoseryl on corneal alkali burn accelerated wound healing with less neovascularization, greater opacity, and less cataract. IHC showed that the inflammation of the cornea was controlled by both the hyaluronic acid and Solcoseryl treatments. On the other hand, the inflammation had spread to the retina. When the dosage forms were compared, eyedrops were more effective on corneal inflammation, while the gel-type had a greater effect on retinal inflammation. Conclusion Solcoseryl was effective in accelerating the wound healing rate on a corneal alkali burn but could not prevent the spread of inflammation from the cornea to the retina. Eyedrops were more effective on inflammation in the cornea, and the gel was more effective in the retina.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1I1A3A0105910212).

References

  1. World Health Organization. World health statistics [Internet]. Geneva: World Health Organization; 2015. [cited 2020 Oct 20]. Available from: https://www.who.int/gho/publications/world_health_statistics/2015/en/.
  2. Whitcher JP, Srinivasan M, Upadhyay MP. Corneal blindness: a global perspective. Bull World Health Organ 2001;79:214-21.
  3. Dua HS, King AJ, Joseph A. A new classification of ocular surface burns. Br J Ophthalmol 2001;85:1379-83. https://doi.org/10.1136/bjo.85.11.1379
  4. Eissa AE, Zaki MM, Saeid S, Abdelsalam M, Ali HM, Moustafa AA, et al. In vitro evaluation of the efficacy of hemodialysate (Solcoseryl®) as a wound healing agent in Nile tilapia (Oreochromis niloticus). Int J Vet Sci Med 2013;1:57-64. https://doi.org/10.1016/j.ijvsm.2013.09.003
  5. Dana MR, Streilein JW. Loss and restoration of immune privilege in eyes with corneal neovascularization. Invest Ophthalmol Vis Sci 1996;37:2485-94.
  6. Bai JQ, Qin HF, Zhao SH. Research on mouse model of grade II corneal alkali burn. Int J Ophthalmol 2016;9:487-90.
  7. Goo H, Kim H, Ahn J, Cho K. Effects of low-level light therapy at 740 nm on dry eye disease in vivo. Med Laser 2019;8:50-8. https://doi.org/10.25289/ML.2019.8.2.50
  8. Wilmink JM, Stolk PW, van Weeren PR, Barneveld A. The effectiveness of the haemodialysate Solcoseryl for second-intention wound healing in horses and ponies. J Vet Med A Physiol Pathol Clin Med 2000;47:311-20. https://doi.org/10.1046/j.1439-0442.2000.00287.x
  9. Jochle W, Hamm D. Effects of a protein-free dialysate of calf blood (Solcoserylrr)(a) and its hexosylceramide fraction on experimental corneal lesions in dogs. Clin Res Regul Aff 1986;4:59-73. https://doi.org/10.3109/10601338609032956
  10. Isler H, Bauen A, Baschong W. Topical treatment of standardized burns with a protein-free haemodialysate. Burns 1991;17:93-7. https://doi.org/10.1016/0305-4179(91)90130-9
  11. Isler H, Bauen A, Hubler M, Oberholzer M. Morphometric assessment of wound healing in rats treated with a protein-free haemodialysate. Burns 1991;17:99-103. https://doi.org/10.1016/0305-4179(91)90131-y
  12. Krupp S, Nadjafi A. [The effect of Solcoseryl on wound heling in dwarf pigs]. Helv Chir Acta 1969;36:634-40. German.
  13. Gupta A, Lakshmi V, Jain GK, Raghubir R. Wound healing in guinea pigs after topical application of starfish Pentaceraster regulus extract. J Wound Care 2008;17:441-4. https://doi.org/10.12968/jowc.2008.17.10.31308
  14. Biland L, Hurlimann F, Goor W, Korner WF, Kundig A, Madar G, et al. Treatment of venous ulcers. A multi-center randomized double-blind study. Vasa 1985;14:383-9.
  15. Rossano C, Weiner M, Haigis E. Clinical, blood gas, and lactate changes in the course of the treatment of peripheral vascular disease with a hemodialysate. Arzneimittelforschung 1990;40:1195-200.
  16. Nam SM, Maeng YS. Wound healing and mucin gene expression of human corneal epithelial cells treated with deproteinized extract of calf blood. Curr Eye Res 2019;44:1181-8. https://doi.org/10.1080/02713683.2019.1633360
  17. Spurr-Michaud S, Argueso P, Gipson I. Assay of mucins in human tear fluid. Exp Eye Res 2007;84:939-50. https://doi.org/10.1016/j.exer.2007.01.018
  18. Blalock TD, Spurr-Michaud SJ, Tisdale AS, Heimer SR, Gilmore MS, Ramesh V, et al. Functions of MUC16 in corneal epithelial cells. Invest Ophthalmol Vis Sci 2007;48:4509-18. https://doi.org/10.1167/iovs.07-0430
  19. Ghate D, Edelhauser HF. Ocular drug delivery. Expert Opin Drug Deliv 2006;3:275-87. https://doi.org/10.1517/17425247.3.2.275
  20. Sultana Y, Jain R, Aqil M, Ali A. Review of ocular drug delivery. Curr Drug Deliv 2006;3:207-17. https://doi.org/10.2174/156720106776359186
  21. Lee MW, Lee TH, Won YK, Shin YI, Kim JY. Characteristics of retinal layer thickness in acute anterior uveitis: an optical coherence tomography study. Acta Ophthalmol 2020;98:e50-5. https://doi.org/10.1111/aos.14221
  22. Kim EK, Park HL, Park CK. Relationship between retinal inner nuclear layer thickness and severity of visual field loss in glaucoma. Sci Rep 2017;7:5543. https://doi.org/10.1038/s41598-017-05282-4
  23. Huang T, Wang Y, Zhang H, Gao N, Hu A. Limbal allografting from living-related donors to treat partial limbal deficiency secondary to ocular chemical burns. Arch Ophthalmol 2011;129:1267-73. https://doi.org/10.1001/archophthalmol.2011.251
  24. Bringmann A, Iandiev I, Pannicke T, Wurm A, Hollborn M, Wiedemann P, et al. Cellular signaling and factors involved in Muller cell gliosis: neuroprotective and detrimental effects. Prog Retin Eye Res 2009;28:423-51. https://doi.org/10.1016/j.preteyeres.2009.07.001
  25. Munk MR, Beck M, Kolb S, Larsen M, Hamann S, Valmaggia C, et al. Quantification of retinal layer thickness changes in acute macular neuroretinopathy. Br J Ophthalmol 2017;101:160-5. https://doi.org/10.1136/bjophthalmol-2016-308367