Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Efficient Constitutive Expression of Cellulolytic Enzymes in Penicillium oxalicum for Improved Efficiency of Lignocellulose Degradation

  • Waghmare, Pankajkumar Ramdas (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Waghmare, Pratima Pankajkumar (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Gao, Liwei (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Sun, Wan (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Qin, Yuqi (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Liu, Guodong (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Qu, Yinbo (State Key Laboratory of Microbial Technology, Shandong University)
  • Received : 2021.01.04
  • Accepted : 2021.03.10
  • Published : 2021.05.28
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Abstract

Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

Keywords

Acknowledgement

This work was supported by National Key R&D Program of China Grant (2018YFA0900500), Major Basic Research Program of Shandong Provincial Natural Science Foundation (ZR2019ZD19), the Key Research and Development Project of Shandong Province (2019JZZY020223 and 2019JZZY020807), the Young Scholars Program of Shandong University (YSPSDU) (to G. L.), and the China/Shandong University International Postdoctoral Exchange Program.

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