Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Safety effect of fermented oyster extract on the endocrine disruptor assay in vitro and in vivo

  • Lee, Hyesook (Department of Biochemistry, College of Korean Medicine, Dong-eui University) ;
  • Hwangbo, Hyun (Department of Biochemistry, College of Korean Medicine, Dong-eui University) ;
  • Ji, Seon Yeong (Department of Biochemistry, College of Korean Medicine, Dong-eui University) ;
  • Oh, Seyeon (Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University) ;
  • Byun, Kyung-A (Functional Cellular Networks Laboratory, College of Medicine, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, Gachon University) ;
  • Park, Joung-Hyun (Ocean Fisheries & Biology Center, Marine Bioprocess Co., Ltd.) ;
  • Lee, Bae-Jin (Ocean Fisheries & Biology Center, Marine Bioprocess Co., Ltd.) ;
  • Kim, Gi-Young (Department of Marine Life Science, Jeju National University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Korean Medicine, Dong-eui University)
  • Received : 2021.09.17
  • Accepted : 2021.09.28
  • Published : 2021.10.31
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Abstract

Oyster (Crassostrea gigas) is a marine bivalve mollusk widely distributed in coastal areas, and have been long widely used in industrial resources. Several studies demonstrated that fermented oyster (FO) extract attribute to bone health, but whether administration of FO play as an endocrine disruptor has not been studied. Therefore, in the present study, we investigated the effect of FO on the endocrine system in vitro and in vivo. As the results of the competitive estrogen receptor (ER) and androgen receptor (AR) binding affinities, FO was not combined with ER-α, ER-β, and AR. However, 17β-estradiol and testosterone, used as positive control, were interacted with ER and AR, respectively. Meanwhile, oral administration of 100 mg/kg and 200 mg/kg of FO doesn't have any harmful effect on the body weight, androgen-dependent sex accessory organs, estrogen-dependent-sex accessory organs, kidney, and liver in immature rats. In addition, FO supplementation has no effect on the serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone, and 17β-estradiol. However, the relative weight of androgen- and estrogen-dependent organs were significantly increased by subcutaneously injection of 4.0 mg/kg of testosterone propionate (TP) and by orally administration of 1.0 ㎍ of 17α-ethynyl estradiol (EE) in immature male and female rats, respectively. Furthermore, TP and EE administration markedly decreased the serum LH and FSH levels, which are similar those of mature Sprague-Dawley (SD) rat. Furthermore, the testosterone and 17β-estradiol levels were significantly enhanced in TP and EE-treated immature rats. Taken together, our findings showed that FO does not interact with ER and AR, suggesting consequentially FO does not play as a ligand for ER and AR. Furthermore, oral administration of FO did not act as an endocrine disruptor including androgenic activity, estrogenic activity, and abnormal levels of sex hormone, indicating FO may ensure the safety on endocrine system to develop dietary supplement for bone health.

Keywords

Acknowledgement

This research was part of the project titled "Development of functional food products with natural materials derived from marine resources" funded by the Ministry of Oceans and Fisheries, Korea (grant no.:20170285).

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