Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Non-Polar Myxococcus fulvus KYC4048 Metabolites Exert Anti-Proliferative Effects via Inhibition of Wnt/β-Catenin Signaling in MCF-7 Breast Cancer Cells

  • Park, Juha (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Yoo, Hee-Jin (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Yu, Ah-Ran (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Kim, Hye Ok (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Park, Sang Cheol (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Jang, Young Pyo (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University) ;
  • Lee, Chayul (Lifetogether Co., Ltd.) ;
  • Choe, Wonchae (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Kim, Sung Soo (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Kang, Insug (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Yoon, Kyung-Sik (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University)
  • Received : 2020.12.08
  • Accepted : 2021.03.16
  • Published : 2021.04.28
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Abstract

The Wnt/β-catenin signaling pathway is involved in breast cancer and Myxococcus fulvus KYC4048 is a myxobacterial strain that can produce a variety of bioactive secondary metabolites. Although a previous study revealed that KYC4048 metabolites exhibit anti-proliferative effects on breast cancer, the biochemical mechanism involved in their effects remains unclear. In the present study, KYC4048 metabolites were separated into polar and non-polar (ethyl acetate and n-hexane) fractions via liquid-liquid extraction. The effects of these polar and non-polar KYC4048 metabolites on the viability of breast cancer cells were then determined by MTT assay. Expression levels of Wnt/β-catenin pathway proteins were determined by Western blot analysis. Cell cycle and apoptosis were measured via fluorescence-activated cell sorting (FACS). The results revealed that non-polar KYC4048 metabolites induced cell death of breast cancer cells and decreased expression levels of WNT2B, β-catenin, and Wnt target genes (c-Myc and cyclin D1). Moreover, the n-hexane fraction of non-polar KYC4048 metabolites was found most effective in inducing apoptosis, necrosis, and cell cycle arrest, leading us to conclude that it can induce apoptosis of breast cancer cells through the Wnt/β-catenin pathway. These findings provide evidence that the n-hexane fraction of non-polar KYC4048 metabolites can be developed as a potential therapeutic agent for breast cancer via inhibition of the Wnt/β-catenin pathway.

Keywords

References

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