융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

부처손 잎 추출물의 항산화, 항염 및 피부재생 효능

Anti-oxidant, Anti-inflammatory, and Wound Healing Activities of Selaginella tamariscina Leaf Extract

  • 김유진 (서경대학교 일반대학원 미용예술학과) ;
  • 이지안 (서경대학교 일반대학원 미용예술학과)
  • Kim, Yu-Jin (Dept. of Beauty Art, Graduate School, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
  • 투고 : 2021.03.16
  • 심사 : 2021.04.20
  • 발행 : 2021.04.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

부처손 잎은 전통한방에서 탈항, 혈뇨, 부정출혈, 월경불순, 자궁출혈 및 만성간염을 치료 목적으로 사용되어 왔다. 본 연구에서는 부처손을 이용하여 얻은 열수추출물(STW)과 80%에탄올추출물(STE)의 항산화, 항염 및 세포 재생활성을 평가하였다. 그 결과 열수추출물과 에탄올추출물의 폴리페놀 화합물 함량은 각각 38.108±0.766 mg/g, 17.927±1.064 mg/g이었다. DPPH와 ABTS radical 소거능 IC50은 열수추출물이 에탄올추출물에 비해 2배 이상 낮았다. MTT 결과, RAW264.7 세포 생존율은 두 추출물 모두 1 mg/mL에서 6% 감소한 반면, HaCaT 세포의 생존율은 50 ㎍/mL에서 18%로 증가하였다. 열수추출물과 에탄올추출물은 LPS로 자극한 RAW264.7 세포의 NO, TNF-�� 그리고 COX-2, PGE2 등의 염증 매개물질의 생성을 억제하였다. 또한 TNF-a에 의한 각질형성 세포의 손상을 저해시켰다. 이상의 결과로 부처손 열수추출물과 에탄올추출물은 피부건강과 미용 식품을 위한 기능성 원료로 가능성이 높다고 사료된다.

The leaves of Selaginella tamariscina were used for the treatment of many diseases in traditional medicine. In the study, antioxidant, anti-inflammatory, and wound healing activities of the hot-water extract(STW) and 80%ethanol extract(STE) obtained from S. tamariscina were evaluated. As a result, the polyphenol content of STW and STE were 38.108±0.766 mg/g and 17.927±1.064 mg/g, respectively. The DPPH and ABTS radical scavenging activities with the IC50 values of the STW were over 2 times lower than that of the STE. In the MTT assay, RAW264.7 cell viability of two extracts was decreased by about 6% at 1 mg/mL, whereas for HaCaT cell viability increased by 18% at 50 ㎍/mL. In addition, STW and STE suppressed the production of nitric oxide(NO), Tumor-necrosis(TNF)-��. COX-2 and PGE2 in lipopolysaccharide(LPS) induced RAW264.7 cells. Furthermore, the STE showed wound healing effect through the promotion of skin cell migration in TNF-�� stimulated human keratinocytes. These results indicated that the STW and STE have the potential to be used as a new cosmetic active ingredients in skin care.

키워드

참고문헌

  1. S. Y. Park & J. D. Kim. (2020). A study on recognition, current use and satisfaction with natural cosmetic products. The Korean Society of Cosmetics and Cosmetology, 10(2), 157-172.
  2. S. J. Chu, J. S. Heo & K. h. Sohn. (2016). Selaginella tamariscina extract improves scopolamine-induced learning and memory deficits in rats. Korean Journal of Pharmacognosy, 47(4), 319-326.
  3. X. K. Zheng et al. (2013). Antihyperlipidaemic and antioxidant effect of the total flavonoids in Selaginella tamariscina (Beauv.) Spring in diabetic mice. Journal of Pharmacy And Pharmacology, 65, 757-766. DOI : 10.1111/jphp.12035
  4. J. h. Hwang, H. Choi, E. R. Woo & D. G. Lee. (2013). Antibacterial effect of amentoflavone and its synergistic effect with antibiotics. Journal of Microbiology and Biotechnology, 23(7), 953-958. DOI : 10.4014/jmb.1302.02045
  5. S. M. Choi, K. Y. Lee, E. J. Jang, S. M. Cha & J. D. Cha. (2019). Antimicrobial activity of Selaginella tamariscina extract against oral bacteria. Dental, Oral and Craniofacial Research, 5, 1-7. DOI : 10.15761/DOCR.1000299
  6. I. S. Lee, A. Nishikawa, F. Furukawa, K. Kasahara & S. U. Kim. (1999). Effects of Selaginella tamariscina on in vitro tumor cell growth, p53 expression, G1 arrest and in vivo gastric cell proliferation. Cancer Letters, 144(1), 93-99. DOI : 10.1016/s0304-3835(99)00202-5
  7. M. S. Liu, C. T. Chien & T. P. Lin. (2008). Constitutive components and induced gene expression are involved in the desiccation tolerance of Selaginella tamariscina. Plant Cell Physiology, 49(4), 653-663. DOI : 10.1093/pcp/pcn040
  8. W. Gu, A. Zhang, H. Sun, Y. Gu, J. Chao, R. Tian & J. A. Duan. (2019). Identifying resurrection genes through the differentially expressed genes between Selaginella tamariscina (Beauv.) spring and Selaginella moellendorffii Hieron under drought stress. PLOS ONE, 10(13), 1-20. DOI : 10.1371/journal.pone.0224765
  9. AOAC. (1980). Official Methods of Analysis. 13 th ed., Association of Official Analytical Chemists. (pp. 376-384). Washington D.C, USA
  10. M. S. Blois. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  11. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. R. Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. DOI : 10.1016/s0891-5849(98)00315-3
  12. A. Murakami, M. Nakashima, T. Koshiba, T. Maoka, H. Nishino, M. Yano, T. Sumida, O. K. Kim, K. Koshimizu and J. Ohigashi. (2000). Modifying effects of carotenoids on superoxide and nitric oxide generation from stimulated leukocytes. Cancer Letters, 149, 115-123. DOI : 10.1016/s0304-3835(99)00351-1
  13. C. C. Liang, A. Y. Park & J. L. Guan. (2007). In Vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature protocols, 2(2), 329-333. DOI : 10.1038/nprot.2007.30
  14. X. K Zheng, Y. J. Li, L. Zhang, W. S. Feng & X. Zhang. (2011). Antihyperglycemic activity of Selaginella tamariscina (Beauv.)Spring. Journal of Ethnopharmacology, 133, 531-537. DOI : 10.1016/j.jep.2010.10.028
  15. D. Sinha. (2019). Pharmacological importance of polyphenols: a review. International Research Journal of Pharmacy, 10(9), 13-23. DOI : 10.7897/2230-8407.1009255
  16. G. H. Kim, S. Y. Lee & A. R. Lee. (2019). The effect of Selaginella tamariscina on inhibition of pancreatic lipase and lipid accumulation. The Korean Journal of Food and Nutrition, 32(1), 27-32. DOI : 10.9799/ksfan.2019.32.1.027
  17. B. C. Lee, G. S. Sim, J. H. Kim, J. H. Kim & H. B. Pyo. (2006). Effect of the processed Selaginella tamariscina on antioxidation and inhibition of Matrix Metalloproteinase. Journal of the Society of Cosmetic Scientists of Korea, 32(2), 69-74.
  18. J. A. Jeong, S. H. Kwon & C. H. Lee. (2007). Screening for antioxidative activities of extracts from aerial and underground parts of some edible and medicinal ferns. Korean Journal of Plant Resources, 20(2), 185-192.
  19. S. Y. Shim, S. G. Lee & M. Lee. (2018). Biflavonoids isolated from Selaginella tamarisicina and their anti-inflammatory activities via ERK 1/2 signaling. Molecules, 23(4), 926-938. DOI : 10.3390/molecules23040926.
  20. C. Cao, M. Yu & Y. Chai. (2019). Pathological alteration and therapeutic implications of sepsis-induced immune cell apoptosis. Cell Death & Disease, 10(10), 782-796. DOI : 10.1038/s41419-019-2015-1
  21. E. R, Woo, J. Y. Lee, I. J. Cho & K. W. Kang. (2005). Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-κB activation in macrophages. Pharmacological Research, 51(6), 539-546. DOI : 10.1016/j.phrs.2005.02.002
  22. Q. Wang, Y. He, Y. Shen, Q. Zhang, D. Chen, C. Zuo, J. Qin, H. Wang, J. Wang & Y. Yu. (2014). Vitamin D Inhibits COX-2 Expression and Inflammatory Response by Targeting Thioesterase Superfamily Member. Journal of Biological Chemistry, 289(17), 11681-11694. DOI : 10.1074/jbc.M113.517581
  23. A. N Won, S. A. Kim, J. Y. Ahn, J. H. Han, C. H. Kim, J. H. Lee & D. I. Kim. (2018). HO-1 induction by Selaginella tamarisicina extract inhibits inflammatory response in lipopolysaccharide-stimulated RAW264.7 macrophages. Evidence-Based Complementary and Alternative Medicine, 2018(11), 1-10. DOI : 10.1155/2018/7816923
  24. V. Prasad & A. K. Dorle. (2006). Evaluation of ghee based formulation for wound healing activity, Journal of Ethnopharmacology, 107(1), 38-47. DOI : 10.1016/j.jep.2006.02.006.
  25. M. J. Jeon et al. (2017). Anti-inflammatory effects and improving atopic dermatitis of ethanol extracts of red sea cucumber. Korean Society for Biotechnology and Biogengineering Journal, 32(4), 335-341. DOI : 10.7841/ksbbj.2017.32.4.335