Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter

  • Lee, Seong Shin (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Paradhipta, Dimas Hand Vidya (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Lee, Hyuk Jun (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Joo, Young Ho (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Noh, Hyeon Tak (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Choi, Jeong Seok (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Ji, Keum Bae (Institute of Technology, Livemac Co. Ltd.) ;
  • Kim, Sam Churl (Division of Applied Life Science (BK21Four, Insti. of Agri. & Life Sci.), Gyeongsang National University)
  • Received : 2020.08.03
  • Accepted : 2020.10.05
  • Published : 2021.06.01
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Abstract

Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents. Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×105 colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p<0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly. Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.

Keywords

References

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