Parasites, Hosts and Diseases

  • 제59권1호
  • /
  • Pages.83-88
  • /
  • 2021
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Parvatrema duboisi (Digenea: Gymnophallidae) Life Cycle Stages in Manila Clams, Ruditapes philippinarum, from Aphae-do (Island), Shinan-gun, Korea

  • Jung, Bong-Kwang (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Chang, Taehee (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Shin, Hyejoo (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Ryoo, Seungwan (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Hong, Sooji (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Jeonggyu (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Song, Hyemi (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Cho, Jaeeun (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Kim, Deok-Gyu (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Jun, Hojong (Department of Tropical Medicine, Inha University College of Medicine) ;
  • Kim, Min-Jae (Department of Infectious Diseases, Asan Medical Center) ;
  • Won, Eun Jeong (Department of Parasitology and Tropical Medicine, Chonnam National University Medical School) ;
  • Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University) ;
  • Shin, Eun-Hee (Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion)
  • 투고 : 2020.12.12
  • 심사 : 2020.12.23
  • 발행 : 2021.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Life cycle stages, including daughter sporocysts, cercariae, and metacercariae, of Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974 (Digenea: Gymnophallidae) have been found in the Manila clam Ruditapes philippinarum from Aphae-do (Island), Shinan-gun, Jeollanam-do, Korea. The daughter sporocysts were elongated sac-like and 307-570 (av. 395) ㎛ long and 101-213 (av. 157) ㎛ wide. Most of the daughter sporocysts contained 15-20 furcocercous cercariae each. The cercariae measured 112-146 (av. 134) ㎛ in total length and 35-46 (av. 40) ㎛ in width, with 69-92 (av. 85) ㎛ long body and 39-54 (av. 49) ㎛ long tail. The metacercariae were 210-250 (av. 231) ㎛ in length and 170-195 (av. 185) ㎛ in width, and characterized by having a large oral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, strongly suggesting P. duboisi. The metacercariae were experimentally infected to ICR mice, and adults were recovered at day 7 post-infection. The adult flukes were morphologically similar to the metacercariae except in the presence of up to 20 eggs in the uterus. The daughter sporocysts and metacercariae were molecularly (ITS1-5.8S rDNA-ITS2) analyzed to confirm the species, and the results showed 99.8-99.9% identity with P. duboisi reported from Kyushu, Japan and Gochang, Korea. These results confirmed the presence of various life cycle stages of P. duboisi in the Manila clam, R. philippinarum, playing the role of the first as well as the second intermediate host, on Aphae-do (Island), Shinan-gun, Korea.

키워드

참고문헌

  1. Scholz T. Family Gymnophallidae Odhner, 1905. In Gibson DI, Jones A, and Bray RA eds, Keys to the Trematoda. Vol. 1. London, UK. Natural History Museum. 2002, pp 245-251.
  2. Chang TH, Jung BK, Shin H, Hong S, Lee J, Kim DG, Patarwut L, Sohn WM, Chai JY. Morphological and molecular confirmation of Parvatrema duboisi metacercariae in the Manila clam Ruditapes philippinarum from Gochang-gun, Korea. Korean J Parasitol 2020; 58: 87-91. https://doi.org/10.3347/kjp.2020.58.1.87
  3. Chai JY, Choi MH, Yu JR, Lee SH. Gymnophalloides seoi: a new human intestinal trematode. Trends Parasitol 2003; 19: 109-112. https://doi.org/10.1016/S1471-4922(02)00068-5
  4. Shin EH, Park JH, Guk SM, Kim JL, Chai JY. Intestinal helminth infections in feral cats and a raccoon dog on Aphae Island, Shinangun, with a special note on Gymnophalloides seoi infection in cats. Korean J Parasitol 2009; 47: 189-191. https://doi.org/10.3347/kjp.2009.47.2.189
  5. Ching HL. Four new species of gymnophallid digeneans from rice rats, willets, and molluscs in Florida. J Parasitol 1995; 81: 924-928. https://doi.org/10.2307/3284042
  6. Lee SH, Chai JY, Hong ST. Gymnophalloides seoi n. sp. (Digenea: Gymnophallidae), the first report of human infection by a gymnophallid. J Parasitol 1993; 79: 677-680. https://doi.org/10.2307/3283602
  7. Yamaguti S. Studies on the helminth fauna of Japan. Part 25. Trematodes of birds IV. Jpn J Zool 1939; 8: 129-210 (in Japanese).
  8. Yu JR, Chai JY, Lee SH. Parvatrema timondavidi (Digenea: Gymnophallidae) transmitted by a clam, Tapes philippinarum, in Korea. Korean J Parasitol 1993; 31: 7-12. https://doi.org/10.3347/kjp.1993.31.1.7
  9. Sohn WM, Na BK, Ryang YS, Ching HL, Lee SH. Parvatrema chaii n. sp. (Digenea: Gymnophallidae) from mice experimentally infected with metacercariae collected from surf-clam, Mactra veneriformis. Korean J Parasitol 2007; 45: 115-120. https://doi.org/10.3347/kjp.2007.45.2.115
  10. Chung OS, Lee HJ, Sohn WM, Park YK, Chai JY, Seo M. Discovery of Parvatrema duboisi and Parvatrema homoeotecnum (Digenea: Gymnophallidae) from migratory birds in Korea. Korean J Parasitol 2010; 48: 271-274. https://doi:10.3347/kjp.2010.48.3.271
  11. Chai JY, Han ET, Choi D, Seo M, Kim JL, Guk SM, Shin EH, Lee SH. A new gymnophallid trematode from the intestine of mice infected with metacercariae from the razor clam Sinonovacula constricta. J Parasitol 2007; 93: 132-137. https://doi.org/10.1645/GE829R2.1
  12. Sohn WM, Chai JY, Lee SH. Infection status of Tapes philippinarum collected from southern coastal areas of Korea with Parvatrema spp. (Digenea: Gymnophallidae) metacercariae. Korean J Parasitol 1996; 34: 273-277 (in Korean). https://doi.org/10.3347/kjp.1996.34.4.273
  13. Sohn WM, Na BK, Cho SH, Lee WJ. Prevalence and density of digenetic trematode metacercariae in clams and oysters from western coastal regions of the Republic of Korea. Korean J Parasitol 2017; 55: 399-408. https://doi.org/10.3347/kjp.2017.55.4.399
  14. Lee SH, Chai JY, Lee HJ, Hong ST, Yu JR, Sohn WM, Kho WG, Choi MH, Lim YJ. High prevalence of Gymnophalloides seoi infection in a village on a southwestern island of the Republic of Korea. Am J Trop Med Hyg 1994; 51: 281-285. https://doi.org/10.4269/ajtmh.1994.51.281
  15. Yanagida T, Shirakashi S, Iwaki T, Ikushima N, Ogawa K. Gymnophallid digenean Parvatrema duboisi uses Manila clam as the first and second intermediate host. Parasitol Int 2009; 58: 308-310. https://doi.org/10.1016/j.parint.2009.05.006
  16. Ching HL. Life cycles of Lacunovermis conspicuus n. gen., n. sp. and Meiogymnophallus multigemmulus n. gen., n. sp. (Gymnophallidae: Trematoda) from Macoma inconspicua and diving ducks from Vancouver, Canada. Proc Helminthol Soc Wash 1965; 32: 53-63.
  17. Yamaguti S. Synopsis of digenetic trematodes of vertebrates. Vol. I. Tokyo, Japan. Keigaku Publishing Co. 1971, pp 511-515.
  18. Ching HL. Evaluation of characters of the digenean family Gymnophallidae Morozov. 1955. Can J Fish Aquat Sci 1995; 52 (suppl): 78-83. https://doi.org/10.1139/f95-511
  19. Ching HL. Paragymnophallus odhneri gen.n., sp. n. (Trematoda: Gymnophallidae) for Gymnophallus somateriae sensu Odhner (1900, 1905). Can J Zool 1973; 51: 807-810. https://doi.org/10.1139/z73-120
  20. Cremonte F, Gilardoni C, Pina S, Rodrigues P, Ituarte C. Revision of the family Gymnophallidae Odhner, 1905 (Digenea) based on morphological and molecular data. Parasitol Int 2015; 64: 202-210. https://doi.org/10.1016/j.parint.2014.12.003
  21. Dollfus RP. Le Trematode des perles de nacre des moules de Provence. CR Acad Sci Paris 1923; 176: 1427-1429 (in French).
  22. Bartoli P. Note preliminaire sur l'anatomie et la biologie de Parvatrema timondavidi n. sp. (Trematoda: Digenea). CR Acad Sci Paris 1963; 273: 518-520 (in French).
  23. Bartoli P. Donnees novelles sur la morphologie et la biologie de Parvatrema timondavidi Bartoli 1963 (Trematoda: Digenea). Ann Parasitol (Paris) 1965; 40: 155-164 (in French).
  24. Bartoli P. Researches sur le Gymnophallidae F. N. Morozov, 1955 (Digenea), parasites d'oiseaux des cotes de Camargue: systematique, biologie et ecologie. These, Universite de Droit, d'Economie et des Sciences d'Aix-Marseille. 1974, pp 1-338.
  25. Ogata T. On the morphology, ecology and life history of an agamodistome parasitic in a bivalve, Paphia (Ruditapes) philippinarum (Adames et Reeve). Sci Rep Tokyo Bunrika Daigaku 1944; 7: 1-24.
  26. Endo T, Hoshina T. Redescription and identification of a gymnophallid trematode in a brackish water clam, Tapes (Ruditapes) philippinarum. Jpn J Parasitol 1974; 23: 73-77.
  27. Shimura S, Yoshinaga T, Wakabayashi H. Two species of marine metacercariae, Parvatrema duboisi (Gymnophallidae) and Proctoeces sp. (Fellodistomidae), in the clam Tapes philippinarum from Lake Hamana, Japan: morphology and level of infection. Fish Pathol (Japan) 1982; 17: 187-194 (in Japanese). https://doi.org/10.3147/jsfp.17.187
  28. Gaevskaya AV. On the biology of Parvatrema duboisi Bartoli, 1963 (Trematoda: Gymnophallidae) in the Black Sea. Parazitologiia 1973; 7: 61-66 (in Russian).
  29. Ozer A, Guneydag S. Seasonality and host-parasite interrelationship of Mytilus galloprovincialis parasites in Turkish Black Sea coasts. J Marine Biol Assoc UK 2015; 95: 1591-1599. https://doi.org/10.1017/s0025315415000740