Journal of Veterinary Science

  • 제22권4호
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  • Pages.53.1-53.13
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  • 2021
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Optimization of adipogenic differentiation conditions for canine adipose-derived stem cells

  • Kim, Jong-Yeon (Department of Food and Nutrition, College of BioNano Technology, Gachon University) ;
  • Park, Eun-Jung (Department of Food and Nutrition, College of BioNano Technology, Gachon University) ;
  • Kim, Sung-Min (Department of Food and Nutrition, College of BioNano Technology, Gachon University) ;
  • Lee, Hae-Jeung (Department of Food and Nutrition, College of BioNano Technology, Gachon University)
  • 투고 : 2021.04.21
  • 심사 : 2021.06.04
  • 발행 : 2021.07.31
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초록

Background: Canine adipose-derived stem cells (cADSCs) exhibit various differentiation properties and are isolated from the canine subcutaneous fat. Although cADSCs are valuable as tools for research on adipogenic differentiation, studies focusing on adipogenic differentiation methods and the underlying mechanisms are still lacking. Objectives: In this study, we aimed to establish an optimal method for adipogenic differentiation conditions of cADSCs and evaluate the role of peroxisome proliferator-activated receptor gamma (PPARγ) and estrogen receptor (ER) signaling in the adipogenic differentiation. Methods: To induce adipogenic differentiation of cADSCs, 3 different adipogenic medium conditions, MDI, DRI, and MDRI, using 3-isobutyl-1-methylxanthine (M), dexamethasone (D), insulin (I), and rosiglitazone (R) were tested. Results: MDRI, addition of PPARγ agonist rosiglitazone to MDI, was the most significantly facilitated cADSC into adipocyte. GW9662, an antagonist of PPARγ, significantly reduced adipogenic differentiation induced by rosiglitazone. Adipogenic differentiation was also stimulated when 17β-estradiol was added to MDI and DRI, and this stimulation was inhibited by the ER antagonist ICI182,780. Conclusions: Taken together, our results suggest that PPARγ and ER signaling are related to the adipogenic differentiation of cADSCs. This study could provide basic information for future research on obesity or anti-obesity mechanisms in dogs.

키워드

과제정보

We are grateful to Prof. Oh-kyeong Kweon (Seoul National University) for providing the cADSCs.

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