Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Serological evidence of West Nile viral infection in archived swine serum samples from Peninsular Malaysia

  • Mohammed, Mohammed Nma (Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia) ;
  • Yasmin, Abd Rahaman (Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia) ;
  • Noraniza, Mohd Adzahan (Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia) ;
  • Ramanoon, Siti Zubaidah (Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia) ;
  • Arshad, Siti Suri (Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia) ;
  • Bande, Faruku (Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Bayero University) ;
  • Mohammed, Hussni O. (Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University)
  • Received : 2020.07.18
  • Accepted : 2021.01.26
  • Published : 2021.05.31
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Abstract

West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs. We tested 80 archived swine serum samples for the presence of WNV antibody and West Nile (WN) viral RNA using ID Screen West Nile Competition Multi-species enzyme-linked immunosorbent assay kits and WNV-specific primers in reverse transcription polymerase chain reaction assays, respectively. A WNV seroprevalence of 62.5% (50/80) at 95% confidence interval (51.6%-72.3%) was recorded, with a significantly higher seroprevalence among young pigs (weaner and grower) and pigs from south Malaysia. One sample was positive for Japanese encephalitis virus antibodies; WN viral RNA was not detected in any of the serum samples.

Keywords

Acknowledgement

The authors express their gratitude to the Department of Veterinary Services (DVS) Malaysia for facilitating the process of WNV notification and reporting to the World Organization for Animal Health (OIE).

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