The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of Molecular Marker to Detect Citrus Melanose Caused by Diaporthe citri from Citrus Melanose-like Symptoms

  • Choi, Cheol-Woo (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jung, Kyung-Eun (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Min-Ju (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yoon, Su-Hyeon (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Suk-Man (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jin, Seong-Beom (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyun, Jae-Wook (Citrus Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • Received : 2021.07.22
  • Accepted : 2021.10.24
  • Published : 2021.12.01
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Abstract

It is difficult to distinguish melanose and melanoses-like symptoms with the naked eye because they appear similar. To accurately detect melanose symptoms caused by Diaporthe citri from melanose-like symptoms, we developed PCR-based specific primers Dcitri by aligning the internal transcribed spacer (ITS) region of D. citri with the ITS of Diaporthe cytosporella, Diaporthe foeniculina, Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria citri, and Fusarium oxysporum found on citrus peel. PCR results showed that the specific product was amplified in D. citri but not in other isolates including, C. gloeosporioides, B. cinerea, A. citri, F. oxysporum. In addition, specific products were observed in melanose symptoms caused by D. citri but not in melanose-like symptoms, such as copper-injury, sunscald, damages by yellow tea thrips, and pink citrus rust mite. Using the Dcitri primers developed in this study, it is expected that melanose caused by D. citri could be accurately distinguished from melanose-like symptoms.

Keywords

Acknowledgement

This work was carried out with the support of the Research Program for the National Institute of Horticultural & Herbal Science (Project No. PJ01506101), Rural Development Administration, Republic of Korea.

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